Removal of digoxin from plasma using monoclonal anti-digoxin antibodies immobilized on agarose
Monoclonal anti-digoxin antibodies (dig-Ab) have been covalently coupled to agarose supports to evaluate them as part of an extracorporeal device for removal of digoxin from the circulation. The agarose supports studied were Sepharose CL-6B, agarose-polyacrolein microsphere (APAM) beads, Bio Gel A-5m and Affi-gel 15 (Bio-Rad). Antibody concentrations between 2 and 4 mg/g gel were coupled to the agarose beads which were then placed in glass columns. Bovine ..cap alpha..-globulin coupled to the agarose supports was used as a control. Binding capacity and affinity of the immobilized antibody were determined by perfusing the dig-Ab agarose beads with a plasma solution containing /sup 3/H-digoxin and various concentrations of digoxin. The binding capacity of the immobilized dig-Ab was 30% of the theoretical value for Sepharose, Bio Gel and Affigel, and 10% of the theoretical value for dig-Ab coupled to APAM beads. The affinity of the immobilized dig-Ab was 10-100 fold less than non-immobilized Ab (3.4 x 10/sup 8/M/sup -1/. The APAM beads showed a significant decrease in binding of digoxin as the flow rate was increased from 0.5 to 5.0 ml/min. These data demonstrate that dig-Ab coupled to agarose and incorporated into a column can be used to remove digoxin from plasma in vitro.
- Research Organization:
- Univ. of Utah, Salt Lake City
- OSTI ID:
- 5220301
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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DIGOXIN
BLOOD CIRCULATION
REMOVAL
MONOCLONAL ANTIBODIES
BIOCHEMICAL REACTION KINETICS
AFFINITY
BLOOD PLASMA
COVALENCE
FLOW RATE
MICROSPHERES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DIGITALIS GLYCOSIDES
DRUGS
GLYCOSIDES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
ORGANIC COMPOUNDS
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques