Phosphoinositide pool sizes and flux in rabbit aortic rings during normoxia and hypoxia

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5220122

The authors wanted to determine if various ATP-dependent reactions in the phosphatidylinositol cycle are inhibited during hypoxia-induced relaxations (PO/sub 2/ 10-20 mmHg, 10 min). Rings, so treated, show decreases in Pcr/Cr/sub T/ but no change in tissue ATP. Flux measurements were made after a 30 min incubation of resting rings with /sup 3/H-myoinositol. Rings were frozen under resting conditions and 5 min after the muscle was contracted by 5.5 ..mu..M norepinephrine. Effects on pool sizes (nmoles/100 nmole total PL) (N=5) are presented. Data indicate (a) PI resynthesis in the e.r. is inhibited during hypoxia, but plasma membrane kinase reactions may be unaffected; (b) the PI cycle may be altered during hypoxia-induced relaxations of vascular smooth muscle.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5220122
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
AORTA
ARTERIES
ATP
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CONTRACTION
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
MUSCLES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RABBITS
SACCHARIDES
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES