Synthesis rates of inositol phospholipids in carbamoylcholine-stimulated canine trachealis muscle

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7035213

Baron, C B; Coburn, R F

The authors have been able to measure the movement of (/sup 3/H)-myoinositol from the cytoplasmic pool into inositol phospholipids and inositol phosphates in muscle strips labeled at rest (30 min). External label was removed and contraction was initiated by addition of carbamoylcholine (CC), 5.5 ..mu..M. At 0 min, control levels of PI, PIP and PIP/sub 2/ were respectively: 706, 1560 and 503 dpm/nmole. Myo-inositol specific activity was constant +/- CC and independent of time. The apparent rate of PI synthesis was 0.025 nmoles myo-inositol incorporated/nmole PI-min. The authors conclude that a high proportion of PI may be linked to PIP and PIP/sub 2/ turnover, since the rates and levels of increases in specific activities are similar.

• Research Organization: Univ. of Pennsylvania School of Medicine, Philadelphia
• OSTI ID: 7035213
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOSYNTHESIS
CATS
CHEMISTRY
CHOLINE
DRUGS
ESTERS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MUSCLES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
QUATERNARY COMPOUNDS
REACTION KINETICS
RESPIRATORY SYSTEM
SYNTHESIS
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES