Phorbol ester-induced inhibition of. beta. -adrenergic - and vasopressin-mediated responses in an established smooth muscle cell line
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5217533
A-10 cells which are derived from embryonic rat thoracic aorta contain a high density of vasopressin receptors and relatively fewer ..beta..-adrenergic receptors. The effects of vasopressin binding to these cells are two-fold: a) inhibition of isoproterenol-stimulated cAMP accumulation, and; b) stimulation of phosphatidyl inositol turnover. Incubation of these cells with phorbol dibutyrate leads to an attenuation of the responses mediated by ..beta..-adrenergic agonist as well as vasopressin. This effect of phorbol ester is concentration- and time-dependent and can be observed as early as five minutes. The inactive phorbol ester (4 ..cap alpha.. phorbol 12,13-didecanoate) is ineffective in inhibiting ..beta..-adrenergic agonist and vasopressin-mediated responses. Since present evidence indicates that the enzyme protein kinase C (PK-C) is involved in both short-term and long-term regulatory processes such as secretion, smooth muscle contraction and cell growth, these data suggest that both ..beta..-adrenergic and vasopressin receptors and/or some mediator(s) of ..beta..-adrenergic and/or vasopressin responses may be phosphorylated by protein kinase C resulting in an attenuated response of these two hormones.
- Research Organization:
- SK and F Labs., Philadelphia, PA
- OSTI ID:
- 5217533
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
- Country of Publication:
- United States
- Language:
- English
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Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5119008
Induction of desensitization by phorbol ester to. beta. -adrenergic agonist stimulation in adenylate cyclase system of rat reticulocytes
Journal Article
·
Wed Jul 16 00:00:00 EDT 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6984984
Differential role of protein kinase C in desensitization of muscarinic receptor induced by phorbol esters and receptor agonists
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6612860
Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENS
CHEMICAL REACTIONS
CONTRACTION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYMES
ESTERS
HORMONES
INHIBITION
INOSITOL
INOSITOLS
KINETICS
MEMBRANE PROTEINS
MONOSACCHARIDES
MUSCLES
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SYMPATHOMIMETICS
TIME DEPENDENCE
TRANSFERASES
VASOPRESSIN
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENS
CHEMICAL REACTIONS
CONTRACTION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYMES
ESTERS
HORMONES
INHIBITION
INOSITOL
INOSITOLS
KINETICS
MEMBRANE PROTEINS
MONOSACCHARIDES
MUSCLES
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SYMPATHOMIMETICS
TIME DEPENDENCE
TRANSFERASES
VASOPRESSIN