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Phorbol ester-induced inhibition of. beta. -adrenergic - and vasopressin-mediated responses in an established smooth muscle cell line

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5217533

A-10 cells which are derived from embryonic rat thoracic aorta contain a high density of vasopressin receptors and relatively fewer ..beta..-adrenergic receptors. The effects of vasopressin binding to these cells are two-fold: a) inhibition of isoproterenol-stimulated cAMP accumulation, and; b) stimulation of phosphatidyl inositol turnover. Incubation of these cells with phorbol dibutyrate leads to an attenuation of the responses mediated by ..beta..-adrenergic agonist as well as vasopressin. This effect of phorbol ester is concentration- and time-dependent and can be observed as early as five minutes. The inactive phorbol ester (4 ..cap alpha.. phorbol 12,13-didecanoate) is ineffective in inhibiting ..beta..-adrenergic agonist and vasopressin-mediated responses. Since present evidence indicates that the enzyme protein kinase C (PK-C) is involved in both short-term and long-term regulatory processes such as secretion, smooth muscle contraction and cell growth, these data suggest that both ..beta..-adrenergic and vasopressin receptors and/or some mediator(s) of ..beta..-adrenergic and/or vasopressin responses may be phosphorylated by protein kinase C resulting in an attenuated response of these two hormones.

Research Organization:
SK and F Labs., Philadelphia, PA
OSTI ID:
5217533
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENS
CHEMICAL REACTIONS
CONTRACTION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYMES
ESTERS
HORMONES
INHIBITION
INOSITOL
INOSITOLS
KINETICS
MEMBRANE PROTEINS
MONOSACCHARIDES
MUSCLES
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SYMPATHOMIMETICS
TIME DEPENDENCE
TRANSFERASES
VASOPRESSIN