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Erythrocyte membrane ATPase and calcium pumping activities in porcine malignant hyperthermia

Journal Article · · Biochem. Med. Metab. Biol.; (United States)
; ; ;
To investigate possible abnormalities in erythrocyte membrane enzyme activities in the pharmacogenetic disorder MH, membrane ATPase activities have been examined in erythrocyte ghosts prepared from red blood cells of MHS and normal swine. While no differences were noted in Mg2+-ATPase activities, the (Na+, K+)-ATPase activity of MHS erythrocyte ghosts was less than that of normal ghosts. Ca2+-ATPase activity exhibited low- and high-affinity Ca2+-binding sites in both types of erythrocyte ghost. While the Km for Ca2+ was greater for normal than for MHS erythrocyte ghosts at the high-affinity Ca2+-binding site, the reverse was true at the low-affinity Ca2+-binding site. Irrespective of the type of calcium binding site occupied, the Vmax for normal erythrocyte ghost Ca2+-ATPase activity was greater than that for MHS ghosts. In the presence of calmodulin, there was now no difference between MHS and normal erythrocyte ghosts in either the Km for Ca2+ or the Vmax of the Ca2+-ATPase activity. To determine if the calcium pumping activity of intact MHS and normal pig erythrocytes differed, calcium efflux from the /sup 45/Ca-loaded erythrocytes was determined; this activity was significantly greater for MHS than for normal erythrocytes. Thus, the present study confirms that there are abnormalities in the membranes of MHS pig red blood cells. However, we conclude that these abnormalities are unlikely to result in an impaired ability of MHS erythrocytes to regulate their cytosolic Ca2+ concentration.
Research Organization:
Stanford Univ. School of Medicine, CA (USA)
OSTI ID:
5209994
Journal Information:
Biochem. Med. Metab. Biol.; (United States), Journal Name: Biochem. Med. Metab. Biol.; (United States) Vol. 38:3; ISSN BMMBE
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BODY TEMPERATURE
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CALMODULIN
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
HYDROLASES
HYPERTHERMIA
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
METALS
NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SWINE
TRACER TECHNIQUES
VERTEBRATES