Role of the multichain IL-2 receptor complex in the control of normal and malignant T-cell proliferation

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.877511· OSTI ID:5209869

Waldmann, T A

Antigen-induced activation of resting T-cells induces the synthesis of interleukin-2 (IL-2), as well as the expression of specific cell surface receptors for this lymphokine. There are at least two forms of the cellular receptors for IL-2, one with a very high affinity and the other with a lower affinity. The authors have identified two IL-2 binding peptides, a 55-kd peptide reactive with the anti-Tac monoclonal antibody, and a novel 75-kd non-Tac IL-2 binding peptide. Cell lines bearing either the p55, Tac, or the p75 peptide along manifested low-affinity IL-2 binding, whereas cell lines bearing both peptides manifested both high- and low-affinity receptors. Fusion of cell membranes from low-affinity IL-2 binding cells bearing the Tac peptide alone with membranes from a cell line bearing the p75 peptide alone generates hybrid membranes bearing high-affinity receptors. They propose a multichain model for the high-affinity IL-2 receptor in which both the Tac and the p75 IL-2 binding peptides are associated in a receptor complex. In contrast to resting T-cells, human T-cell lymphotropic virus I-associated adult T-cell leukemia cells constitutively express large numbers of IL-2 receptors. Because IL-2 receptors are present on the malignant T-cells but not on normal resting cells, clinical trials have been initiated in which patients with adult T-cell leukemia are being treated with either unmodified or toxin-conjugated forms of anti-Tac monoclonal antibody directed toward this growth factor receptor. Cross-linking studies were done using (/sup 125/I) IL-2.

• Research Organization: National Institutes of Health, Bethesda, MD (USA)
• OSTI ID: 5209869
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 75; ISSN EVHPA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
HEMIC DISEASES
IMMUNOTHERAPY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
SOMATIC CELLS
THERAPY