Interlaboratory study of blood selenium determinations

Journal Article · · Journal of the Association of Official Analytical Chemists; (USA)

OSTI ID:5209804

Koh, Tee-Siaw ^[1]

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1. South Australia Dept. of Agriculture, Adelaide (Australia)

Fifty-one laboratories from 14 countries participated in a survey on the determination of selenium (Se) in 8 bovine samples with Se concentrations ranging from 0.2 {mu}mol/L (0.016 {mu}g/mL) to 14 {mu}mol/L (1.1 {mu}g/mL). The methods used (and the percentage of participants using each method) were fluorometry (61), hydride-generation atomic absorption spectrophotometry (AAS) (23), graphite-furnace AAS (6), gas chromatography (4), neutron activation analysis (4), and X-ray fluorometry (2). There was little difference in the mean Se results obtained by fluorometry or hydride-generation AAS. Mean intralaboratory coefficients of variation (CVs) from known replicates ranged from 4 to 14% for all samples. Interlaboratory CVs were related to blood Se concentration and increased to 55% at Se levels below 0.4 {mu}mol/L (0.032 {mu}g/mL). Laboratories that used quality control (QC) schemes had lower interlaboratory CVs that those that did not, but the advantage began to diminish at blood Se concentration below 0.4 {mu}mol/L (0.032 {mu}g/mL). The high interlaboratory CVs, coupled with the false assurance from the low intralaboratory CVs and the ineffectiveness of the QC schemes at blood SE concentrations below 0.4 {mu}mol/L (0.032 {mu}g/mL), are of concern in diagnosis of marginal Se deficiency in livestock where the concentrations of interest are in the range 0.15-0.5 {mu}mol/L (0.012-0.039 {mu}g/mL).

• OSTI ID: 5209804
• Journal Information: Journal of the Association of Official Analytical Chemists; (USA), Journal Name: Journal of the Association of Official Analytical Chemists; (USA) Vol. 70:4; ISSN 0004-5756; ISSN JANCA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ABSORPTION SPECTROSCOPY
ACTIVATION ANALYSIS
ANIMALS
BARYON REACTIONS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CATTLE
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONTROL
DOMESTIC ANIMALS
ELEMENTS
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
GAS CHROMATOGRAPHY
HADRON REACTIONS
INTERLABORATORY COMPARISONS
MAMMALS
MATERIALS
NEUTRON REACTIONS
NONDESTRUCTIVE ANALYSIS
NUCLEAR REACTIONS
NUCLEON REACTIONS
QUALITY CONTROL
QUANTITATIVE CHEMICAL ANALYSIS
RUMINANTS
SELENIUM
SEMIMETALS
SEPARATION PROCESSES
SPECTROSCOPY
VARIATIONS
VERTEBRATES
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS