Intracellular free calcium concentration measured with /sup 19/F NMR spectroscopy in intact ferret hearts

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.6005· OSTI ID:5207938

Marban, E; Kitakaze, M; Kusuoka, H; Porterfield, J K; Yue, D T; Chacko, V P

Changes in the intracellular free Ca/sup 2 +/ concentration, (Ca/sup 2 +/)/sub i/, mediate excitation-contraction coupling in the heart and contribute to cellular injury during ischemia and reperfusion. To study these processes directly, the authors measured (Ca/sup 2 +/)/sub i/ in perfused ferret (Mustela putorius furo) hearts using /sup 19/F NMR spectroscopy to detect the 5,5'-difluoro derivative of the Ca/sup 2 +/ chelator, 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). To load cells, hearts were perfused with the acetoxymethyl ester derivative of 5,5'-F/sub 2/-BAPTA. They measured /sup 19/F NMR spectra and left ventricular pressure simultaneously,at rest and during pacing at various external Ca concentrations ((Ca)/sub 0/). Although contractile force was attenuated by the Ca/sup 2 +/ buffering properties of 5,5'-F/sup 2/-BAPTA, the decrease in pressure could be overcome by raising (Ca)/sub 0/. The mean value of 104 nM for (Ca/sup 2 +/)/sub i/ at rest in the perfused heart agrees well with previous measurements in isolated ventricular muscle. During pacing at 0.6-4 Hz, time-averaged (Ca/sup 2 +/)/sub i/ increased; the effect of pacing was augmented by increasing (Ca)/sub 0/. (Ca/sup 2 +/)/sub i/ more than tripled during 10-20 min of global ischemia, and returned toward control levels upon reperfusion. This approach promises to be particularly useful in investigating the physiology of intact hearts and the pathophysiology of alterations in the coronary circulation

• Research Organization: Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)
• OSTI ID: 5207938
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:16; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
BIOCHEMISTRY
BODY
CALCIUM COMPOUNDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
CHEMISTRY
DISEASES
FLUORINE 19
FLUORINE ISOTOPES
HEART
IONS
ISCHEMIA
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PERFUSED ORGANS
PHYSIOLOGY
RESONANCE
STABLE ISOTOPES
VASCULAR DISEASES