Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants
Chlorate-resistant Nicotiana plumbaginifolia (cv. Viviani) mutants were found to be defective in the nitrate reductase apoprotein (NR/sup -/ nia). Because they could not grow with nitrate as sole nitrogen source, they were cultivated as graftings on wild type Nicotiana tabacum. The grafts of NR/sup -/ plants were found to contain less malate but more amino acids, sugars and starch than the wild type. Moreover they were chlorotic, with a slight increase of the proportion of LH Chl a/b protein complexes and they exhibited a lowering of the efficiency of energy transfer between the light-harvesting complexes and the active centers. After /sup 14/CO/sub 2/ pulse and chase experiments. The total /sup 14/C incorporation of the mutant leaves was approximately 20% of that of the control. The NR/sup -/ leaves mainly accumulated /sup 14/C in the whole intermediates of the Calvin-cycle and in sucrose. In the most deficient NR leaves, chloroplasts were stuffed with large starch inclusions disorganizing the lamellar system.
- Research Organization:
- I.N.R.A., Versailles, France
- OSTI ID:
- 5207435
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14
BIOLOGICAL ACCUMULATION
NICOTIANA
ENZYMES
MUTANTS
OXIDOREDUCTASES
PHYSIOLOGY
NITRATES
REDUCTION
AMINO ACIDS
BIOCHEMISTRY
CHLORATES
LEAVES
RADIOISOTOPES
SACCHARIDES
STARCH
TOBACCO
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
CHLORINE COMPOUNDS
EVEN-EVEN NUCLEI
HALOGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
NITROGEN COMPOUNDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
REAGENTS
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques