Uptake of Ca/sup 2 +/ and Mn/sup 2 +/ by rat liver submitochondrial particles. [/sup 45/Ca and /sup 54/Mn tracers]

Conference · Sun Jan 01 04:00:00 EST 1978

OSTI ID:5206625

Gunter, T E; Rosier, R N; Tucker, D A; Gunter, K K

Submitochondrial particles (SMPs) were made by sonicating mitoplasts in the presence of cytochrome c and then washing the resulting particles in a 125 mM KCl wash. /sup 45/Ca/sup 2 +/ and /sup 54/Mn/sup 2 +/ uptake into selectively energized SMPs (inside out or right side out) was determined using filtration techniques and under the proper conditions was significant in all cases. No uptake occurs in the absence of substrate or in the presence of cyanide, uncouplers (or other agents which break down the membrane potential), N ethyl maleimide or mersalyl. Ca/sup 2 +/ uptake is small without the addition of inorganic phosphate and either ATP or ADP. Low levels of CCCP enhance Ca/sup 2 +/ uptake in right side out SMPs but inhibit uptake in inside out SMPs consistent with results on mitochondrial Ca/sup 2 +/ efflux.

• Research Organization: Rochester Univ., N.Y. (USA). Dept. of Radiation Biology and Biophysics
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 5206625
• Report Number(s): UR-3490-1237; CONF-7709108-1
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ADP
ALKALINE EARTH METALS
ANIMALS
ATP
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
ISOTOPE APPLICATIONS
KINETICS
LIVER
MAMMALS
MEMBRANE TRANSPORT
METALS
MITOCHONDRIA
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES