Operability and feasibility of ethanol production by immobilized Zymomonas mobilis in a fluidized-bed bioreactor

Conference · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5205923

Davison, B H; Scott, C D

Studies have been carried out using immobilized Z. mobilis in fluidized-bed bioreactors and have emphasized operation during high productivity and conversion. The bacteria are immobilized within small uniform beads (/approximately/1 to 1.5-mm-diam) of kappa-carrageenan at cell loadings of 15 to 50 g (dry wt)/L. Conversion and productivity were measured under a variety of conditions, including feedstocks, flow rates, and column sizes (up to 2.5 m tall). Volumetric productivities of 50 to 100 g EtOH/L/center dot/h have been achieved with residual glucose concentrations of less than 0.1% in the tallest fermentor. Among feeds of 10, 15, and 20% dextrose, the 15% gave the highest productivity and avoided substrate inhibition. The ethanol yield was shown to be nearly constant at 0.49 g EtOH/g glucose or 97% of the theoretical under a variety of conditions and transients. The biocatalyst beads have been shown to remain active for two months. Nonsterile feed has been used for weeks without detrimental contamination. The advantages of this advanced bioreactor systems over conventional batch technology are discussed. 25 refs., 6 figs., 2 tabs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5205923
• Report Number(s): CONF-870523-7; ON: DE88007252
• Resource Relation: Conference: 9. symposium on biotechnology for fuels and chemicals, Boulder, CO, USA, 5 May 1987
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ETHANOL
BIOSYNTHESIS
IMMOBILIZED CELLS
PERFORMANCE TESTING
EXPERIMENTAL DATA
FEASIBILITY STUDIES
FERMENTATION
FLUIDIZED BED REACTORS
ZYMOMONAS MOBILIS
ALCOHOLS
BACTERIA
BIOCONVERSION
DATA
FUEL DISPERSION REACTORS
HOMOGENEOUS REACTORS
HYDROXY COMPOUNDS
INFORMATION
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
REACTORS
SYNTHESIS
TESTING
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)