Liquid chromatography/Fourier transform IR spectrometry interface flow cell

Patent · Fri Jan 04 04:00:00 EST 1985

OSTI ID:5204823

Johnson, C C; Taylor, L T

A zero dead volume (ZDV) microbore high performance liquid chromatography (..mu.. HPLC)/Fourier transform infrared (FTIR) interface flow cell includes an IR transparent crystal having a small diameter bore therein through which a sample liquid is passed. The interface flow cell further includes a metal holder in combination with a pair of inner, compressible seals for directly coupling the thus configured spectrometric flow cell to the outlet of a ..mu.. HPLC column end fitting to minimize the transfer volume of the effluents exiting the ..mu.. HPLC column which exhibit excellent flow characteristics due to the essentially unencumbered, open-flow design. The IR beam passes transverse to the sample flow through the circular bore within the IR transparent crystal, which is preferably comprised of potassium bromide (KBr) or calcium fluoride (CaF/sub 2/), so as to minimize interference patterns and vignetting encountered in conventional parallel-plate IR cells. The long IR beam pathlength and lensing effect of the circular cross-section of the sample volume in combination with the refractive index differences between the solvent and the transparent crystal serve to focus the IR beam in enhancing sample detection sensitivity by an order of magnitude.

• Research Organization: Virginia Polytechnic Inst. and State Univ., Blacksburg (USA)
• DOE Contract Number: FG22-81PC40799
• Assignee: Dept. of Energy
• Patent Number(s): None
• Application Number: ON: DE85017749
• OSTI ID: 5204823
• Country of Publication: United States
• Language: English

Related Subjects

440300* -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BROMIDES
BROMINE COMPOUNDS
CALCIUM COMPOUNDS
CALCIUM FLUORIDES
CALCIUM HALIDES
CHROMATOGRAPHY
FLUORIDES
FLUORINE COMPOUNDS
FOURIER TRANSFORM SPECTROMETERS
HALIDES
HALOGEN COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MEASURING INSTRUMENTS
POTASSIUM BROMIDES
POTASSIUM COMPOUNDS
SEPARATION PROCESSES
SPECTROMETERS