Liquid chromatography/Fourier transform IR spectrometry interface flow cell
A zero dead volume (ZDV) microbore high performance liquid chromatography (..mu.. HPLC)/Fourier transform infrared (FTIR) interface flow cell includes an IR transparent crystal having a small diameter bore therein through which a sample liquid is passed. The interface flow cell further includes a metal holder in combination with a pair of inner, compressible seals for directly coupling the thus configured spectrometric flow cell to the outlet of a ..mu.. HPLC column end fitting to minimize the transfer volume of the effluents exiting the ..mu.. HPLC column which exhibit excellent flow characteristics due to the essentially unencumbered, open-flow design. The IR beam passes transverse to the sample flow through the circular bore within the IR transparent crystal, which is preferably comprised of potassium bromide (KBr) or calcium fluoride (CaF/sub 2/), so as to minimize interference patterns and vignetting encountered in conventional parallel-plate IR cells. The long IR beam pathlength and lensing effect of the circular cross-section of the sample volume in combination with the refractive index differences between the solvent and the transparent crystal serve to focus the IR beam in enhancing sample detection sensitivity by an order of magnitude.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States)
- DOE Contract Number:
- FG22-81PC40799
- Assignee:
- Dept. of Energy
- Application Number:
- ON: DE85017749
- OSTI ID:
- 5204823
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIQUID COLUMN CHROMATOGRAPHY
FOURIER TRANSFORM SPECTROMETERS
CALCIUM FLUORIDES
POTASSIUM BROMIDES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BROMIDES
BROMINE COMPOUNDS
CALCIUM COMPOUNDS
CALCIUM HALIDES
CHROMATOGRAPHY
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
MEASURING INSTRUMENTS
POTASSIUM COMPOUNDS
SEPARATION PROCESSES
SPECTROMETERS
440300* - Miscellaneous Instruments- (-1989)