Superoxide dismutase (SOD) and mineral levels in copper deficient (CuD) and copper adequate (CuA) rat brains

Conference · Thu Mar 14 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5202733

Askari, A; Orlowski, E; Thomford, N R; Birkman, R H ^[1]

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1. Medical College of Ohio, Toledo (United States)

Since copper nutriture affects central nervous tissue and neurological functions, this study was undertaken to assess levels of Cu/ZnSOD, MnSOD, Cu, Zn, Mn, Fe, Mg, Na, K, and Ca in brains from CuD and CuA rats. Male Sprague-Dawley weanling rats were fed ad libitum diets either CuD at 0.6 mg Cu/Kg or CuA at 6.0 mg Cu/kg for 8 weeks. Brain tissues were analyzed for protein content by Lowry method for SOD by pyrogallol autooxidation and KCN quenching. CuD and CuA brains had similar protein, Cu/ZnSOD and MnSOD. Brain specimens were acid digested before mineral analysis by atomic absorption. Only Cu and K were different. Thus, brains from CuD rats maintain normal mineral levels for Zn, Mn, Fe, Mg, Na, Ca. The SOD defenses against free radicals are supported even though brain Cu is depressed. Depending on its intra/extracellular distribution, elevated K in CuD brain may alter neuron or glial cell functions.

• OSTI ID: 5202733
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTRA
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CALCIUM
CENTRAL NERVOUS SYSTEM
COPPER
DIET
ELEMENTS
ENZYMES
IRON
MAGNESIUM
MAMMALS
MANGANESE
METABOLISM
METALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
POTASSIUM
PROTEINS
RADICALS
RATS
RODENTS
SODIUM
SPECTRA
SUPEROXIDE DISMUTASE
TRANSITION ELEMENTS
VERTEBRATES
ZINC