Radioimmunoimaging in malignant melanoma with /sup 111/In-labeled monoclonal antibody 96. 5

Journal Article · Wed May 01 04:00:00 EDT 1985 · Cancer Res.; (United States)

OSTI ID:5200118

Murray, J L; Rosenblum, M G; Sobol, R E; Bartholomew, R M; Plager, C E; Haynie, T P; Jahns, M F; Glenn, H J; Lamki, L; Benjamin, R S

A radiolabeled monoclonal antibody (96.5) reactive with an Mr 97,000 antigen found on over 80% of melanoma cell lines and tissue extracts was examined for its ability to detect malignant melanoma metastases in vivo. For imaging purposes, it was conjugated with diethyltriaminepentaacetic acid and subsequently labeled with /sup 111/In by chelation. Thirty-one patients with metastatic melanoma received single injections of monoclonal antibody 96.5 at concentrations ranging from 0.5 to 20 mg and at specific activities of /sup 111/In ranging from 0.125 to 4 mCi/mg. Total-body scans were performed at various time intervals following administration. No serious side effects were observed. Of a total of 100 previously documented metastatic sites, 50 imaged for a specificity of 50%. The number of sites imaged increased significantly as the amount of antibody administered increased relative to the average radiation dose. Considerable background uptake of isotope was observed in blood pool and other organs with gradual acquisition of label in tumor sites by 48 to 72 h. Hence, tumor imaging of melanoma using /sup 111/In-labeled monoclonal antibody 96.5 appeared feasible, especially at antibody doses above 2 mg.

• Research Organization: Univ. of Texas System Cancer Center, Houston
• OSTI ID: 5200118
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 5; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETIC ACID
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MELANOMAS
METASTASES
MICE
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
WHOLE-BODY COUNTING