Accelerated recovery of hematopoiesis following sub-lethal whole body irradiation with recombinant murine interleukin-1 (IL-1)

Journal Article · Tue Mar 01 04:00:00 EST 1988 · J. Leukocyte Biol.; (United States)

OSTI ID:5198262

Gallicchio, V S

This communication reports the results of studies designed to investigate the ability of recombinant murine interleukin-1 (rIL-1) to enhance the recovery of hematopoiesis following administration of sub-lethal whole body irradiation (2 Gy). Mice were administered rIL-1 (100 and 500 units) i.p. Twenty-four hours later these mice were administered 2 Gy radiation. Irradiated control mice were given only phosphate buffered saline (PBS). Animals were then serially sacrificed (on days 1, 2, 4, 7, 9, and 12 following irradiation) and their peripheral blood was analyzed for indices (packed red cell volume, WBC, platelets, and differential). Femoral bone marrow was harvested and assayed for their stem cell content--erythroid (CFU-E, BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-MEG). Irradiated mice pretreated with rIL-1 demonstrated accelerated hematopoietic recovery as measured by higher WBC, platelets and femoral stem cell content than PBS-treated irradiated controls. These results indicate IL-1 may be an effective radioprotective agent against the hematotoxicity induced by ionizing radiation.

• Research Organization: Univ. of Kentucky Medical Center, Lexington (USA)
• OSTI ID: 5198262
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 43:3; ISSN JLBIE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
EXTERNAL IRRADIATION
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
IRRADIATION
LYMPHOKINES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SOMATIC CELLS
STEM CELLS
SUBLETHAL IRRADIATION
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION