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Discovery of an agent in wastewater sludge that reduces the heat required to inactivate reovirus

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:5193785
An agent that causes heat inactivation of reovirus to occur at reduced temperatures has been found in both raw and anaerobically digested sludge. This agent is originally associated with sludge solids but can be washed from these solids by blending with water. The activity of the agent was considerably greater in alkaline than in acid solutions, probably because it is insoluble at low pH. The agent was shown to be nonvolatile and heat stable up to 300/sup 0/C but was inactivated within 30 min at 400/sup 0/C. The rate of heat inactivation of reovirus by the agent was found to occur in a bimodal fashion and to be relatively rapid, even at 35/sup 0/C. Finally, it was found that this agent did not accelerate heat inactivation of poliovirus, but instead may be the component of sludge previously found to protect poliovirus against inactivation by heat.
Research Organization:
Sandia Labs., Albuquerque, NM
OSTI ID:
5193785
Journal Information:
Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 34:6; ISSN AEMID
Country of Publication:
United States
Language:
English

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