ECK, a human EPH-related gene, maps to 1p36.1, a common region of alteration in human cancers

Journal Article · Sat Mar 01 00:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4569· OSTI ID:518489

Sulman, E P; Brodeur, G M; Ikegaki, N ^[1]

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1. Children`s Hospital of Philadelphia, PA (United States); and others

Mouse eck, a member of the EPH gene family, has been mapped to mouse chromosome 4. The syntenic relationship between this chromosome and human chromosome 1 suggests that the human ECK gene maps to the distal short arm of human chromosome 1 (1p). Since this region is frequently deleted or altered in certain tumors of neuroectodermal origin, it is important to define the specific chromosomal localization of the human ECK gene. PCR screening of a rodent-human somatic cell hybrid panel by ECK-specific primers showed that ECK is indeed localized to human chromosome 1. Additional PCR screening of a regional screening panel for chromosome 1p indicated that ECK is localized to 1p36, distal to FUCA1. Furthermore, fluorescence in situ hybridization analysis with an ECK-specific P1 clone showed that ECK maps proximal to genetic marker D1S228. Taken together, the data suggest that ECK maps to 1p36.1, a region that is frequently deleted in neuroblastoma, melanoma, and other neuroectodermal tumors. 23 refs., 3 figs.

• OSTI ID: 518489
• Journal Information: Genomics, Vol. 40, Issue 2; Other Information: PBD: 1 Mar 1997
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
PHOSPHOTRANSFERASES
RECEPTORS
GENES
DNA SEQUENCING
GENETIC MAPPING
GENE MUTATIONS
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA-CLONING
HUMAN CHROMOSOME 1
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
MELANOMAS
ETIOLOGY
TYROSINE
NEOPLASMS
AMINO ACID SEQUENCE
MICE
POLYMERASE CHAIN REACTION
SOMATIC CELLS
HYBRIDIZATION
BIOLOGICAL MARKERS
IN-SITU HYBRIDIZATION
FLUORESCENCE
HEREDITARY DISEASES