ECK, a human EPH-related gene, maps to 1p36.1, a common region of alteration in human cancers
- Children`s Hospital of Philadelphia, PA (United States); and others
Mouse eck, a member of the EPH gene family, has been mapped to mouse chromosome 4. The syntenic relationship between this chromosome and human chromosome 1 suggests that the human ECK gene maps to the distal short arm of human chromosome 1 (1p). Since this region is frequently deleted or altered in certain tumors of neuroectodermal origin, it is important to define the specific chromosomal localization of the human ECK gene. PCR screening of a rodent-human somatic cell hybrid panel by ECK-specific primers showed that ECK is indeed localized to human chromosome 1. Additional PCR screening of a regional screening panel for chromosome 1p indicated that ECK is localized to 1p36, distal to FUCA1. Furthermore, fluorescence in situ hybridization analysis with an ECK-specific P1 clone showed that ECK maps proximal to genetic marker D1S228. Taken together, the data suggest that ECK maps to 1p36.1, a region that is frequently deleted in neuroblastoma, melanoma, and other neuroectodermal tumors. 23 refs., 3 figs.
- OSTI ID:
- 518489
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 40; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA SEQUENCING
DNA-CLONING
ETIOLOGY
FLUORESCENCE
GENE MUTATIONS
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 1
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MELANOMAS
MICE
NEOPLASMS
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RECEPTORS
SOMATIC CELLS
STRUCTURE-ACTIVITY RELATIONSHIPS
TYROSINE