Detector for liquid chromatography and flow injection analysis using surface-enhanced Raman spectroscopy

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Appl. Spectrosc.; (United States)

DOI:https://doi.org/10.1366/0003702884427997· OSTI ID:5183345

Freeman, R D; Hammaker, R M; Meloan, C E; Fateley, W G

A successful interface between a high-performance liquid chromatograph and a Raman spectrometer is described. Surface-enhanced techniques are utilized to overcome the sensitivity problem inherent to conventional Raman spectrometry by adding a Ag sol to the chromatographic effluent in a post-column mixing coil. The system is designed so that Raman spectra may be obtained from chromatographic effluent or from flow injection analysis effluent. A common organic dye (pararosaniline hydrochloride) is used to evaluate the reproducibility, dynamic range, and analytical capabilities of the system. The SERS instrument is found to be a viable detector for HPLC and FIA, capable of providing structural information with a sensitivity comparable to that of other commonly used HPLC detectors.

• Research Organization: Kansas State University, Department of Chemistry, Willard Hall, Manhattan, Kansas 66506
• OSTI ID: 5183345
• Journal Information: Appl. Spectrosc.; (United States), Vol. 42:3
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
DYES
LIQUID COLUMN CHROMATOGRAPHY
RAMAN SPECTROSCOPY
EQUIPMENT INTERFACES
FLUID INJECTION
QUANTITATIVE CHEMICAL ANALYSIS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
LASER SPECTROSCOPY
SEPARATION PROCESSES
SPECTROSCOPY
400102* - Chemical & Spectral Procedures