Mutations in GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, suppress the temperature-sensitive growth of hpr1 mutants

Journal Article · Mon Apr 01 04:00:00 EST 1996 · Genetics

OSTI ID:518182

Uemura, Hiroshi; Jigami, Yoshifumi ^[1]; Pandit, Sunil; Sternglanz, R ^[2]

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1. National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki (Japan)
2. State Univ. of New York, Stony Brook, NY (United States)

To study the functions of DNA topoisomerase I and Hpr1 protein, a suppressor mutant of the temperature-sensitive growth of an hpr1 top1-5{sup ts} double mutant was isolated. The isolated triple mutant showed cold-sensitive growth. By complementation of this phenotype, the suppressor gene was cloned. DNA sequencing showed it to be GCR3, a gene involved in the expression of glycol genes. Further analysis showed that gcr3 mutations also suppressed the temperature-sensitive growth of hpr1 single mutants. Experiments with gcr3 truncation mutants also suggested a genetic interaction between GCR3 and HPR1. The fact that top1 suppressed the growth defect of gcr3 suggested an interaction between those two genes also. Plasmid DNA isolated from gcr3 mutants was significantly more negatively supercoiled than normal, suggesting that Gcr3 protein, like topoisomerase I and Hpr1p, affects chromatin structure, perhaps during transcription. 43 refs., 2 figs., 6 tabs.

• OSTI ID: 518182
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 4 Vol. 142; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMATIN
DNA
DNA SEQUENCING
DNA-CLONING
ENZYME ACTIVITY
ENZYMES
GENE MUTATIONS
GENE REGULATION
GENES
GENOTYPE
GLYCOLS
GROWTH
MUTANTS
PHENOTYPE
PLASMIDS
PROTEINS
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
STRUCTURE-ACTIVITY RELATIONSHIPS
TEMPERATURE DEPENDENCE
TRANSCRIPTION