Isolation of pathogenic Naegleria from artificially heated waters
Conference
·
OSTI ID:5180086
Investigations were undertaken to determine whether heated waters facilitate the proliferation of free-living amoeba that cause primary amoebic meningoencephalitis. Water samples were taken close to the discharges of power plants situated on lakes or rivers in Florida and Texas and from cooling towers in Tennessee. The water temperatures ranged from 29 to 42/sup 0/C. Water samples were also taken from several lakes in Florida and Texas without associated power plants. The water temperatures of these ranged from 30/sup 0/ to 34/sup 0/C. Twenty-five-250-ml samples were filtered through membranes. Samples taken from the control lakes and cooling towers showed no growth of pathogenic amoeba, whereas growth was obtained from 2 of the 8 lakes and rivers in Florida and from 1 of the 7 man-made lakes in Texas that were artificially heated. The amoebae were identified as belonging to the genus Naegleria from their trophozoite and cyst structure, ability to grow at 45/sup 0/C, to transform into flagellates, and to produce primary amebic meningoencephalitis (PAME) in mice after intranasal instillation. Their identification as N. fowleri was confirmed by indirect immunofluorescent analysis with antiserum produced against N. fowleri. These findings indicate that artificial heating of waters may facilitate the growth of pathogenic free living amoeba.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5180086
- Report Number(s):
- CONF-770961-3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation of the etiological agent of primary amoebic meningoencephalitis from artificially heated waters
Thermal ecology of Naegleria fowleri from a power plant cooling reservoir
Effect of thermal additions on the density and distribution of thermophilic amoebae and pathogenic Naegleria fowleri in a newly created cooling lake
Journal Article
·
Wed Nov 30 23:00:00 EST 1977
· Appl. Environ. Microbiol.; (United States)
·
OSTI ID:5205306
Thermal ecology of Naegleria fowleri from a power plant cooling reservoir
Journal Article
·
Sun Jul 01 00:00:00 EDT 1990
· Applied and Environmental Microbiology; (USA)
·
OSTI ID:5890905
Effect of thermal additions on the density and distribution of thermophilic amoebae and pathogenic Naegleria fowleri in a newly created cooling lake
Journal Article
·
Tue Feb 28 23:00:00 EST 1989
· Applied and Environmental Microbiology; (USA)
·
OSTI ID:5099015
Related Subjects
520400* -- Environment
Aquatic-- Thermal Effluents Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560202 -- Thermal Effects-- Microorganisms-- (1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMOEBA
ANIMALS
AQUATIC ECOSYSTEMS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
COOLING TOWERS
ECOSYSTEMS
ENVIRONMENTAL EFFECTS
FLORIDA
GENETIC VARIABILITY
GROWTH
HABITAT
INVERTEBRATES
LAKES
MICROORGANISMS
NORTH AMERICA
PATHOGENESIS
PROTOZOA
RIVERS
STREAMS
SURFACE WATERS
TEMPERATURE EFFECTS
TENNESSEE
TEXAS
THERMAL EFFLUENTS
USA
Aquatic-- Thermal Effluents Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560202 -- Thermal Effects-- Microorganisms-- (1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMOEBA
ANIMALS
AQUATIC ECOSYSTEMS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
COOLING TOWERS
ECOSYSTEMS
ENVIRONMENTAL EFFECTS
FLORIDA
GENETIC VARIABILITY
GROWTH
HABITAT
INVERTEBRATES
LAKES
MICROORGANISMS
NORTH AMERICA
PATHOGENESIS
PROTOZOA
RIVERS
STREAMS
SURFACE WATERS
TEMPERATURE EFFECTS
TENNESSEE
TEXAS
THERMAL EFFLUENTS
USA