Effect of thermal additions on the density and distribution of thermophilic amoebae and pathogenic Naegleria fowleri in a newly created cooling lake

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5099015

Tyndall, R L; Ironside, K S; Metler, P L; Tan, E L ^[1]; Hazen, T C; Fliermans, C B ^[2]

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1. Univ. of Tennessee, Knoxville (USA)
2. E.I. du Pont de Nemours and Co., Inc., Aiken, SC (USA)

Pathogenic Naegleria fowleri is the causative agent of fatal human amoebic meningoencephalitis. The protozoan is ubiquitous in nature, and its presence is enhanced by thermal additions. In this investigation, water and sediments from a newly created cooling lake were quantitatively analyzed for the presence of thermophilic amoebae, thermophilic Naegleria spp., and the pathogen Naegleria fowleri. During periods of thermal additions, the concentrations of thermophilic amoebae and thermophilic Naegleria spp. increased as much as 5 orders of magnitude, and the concentration of the pathogen N. fowleri increased as much as 2 orders of magnitude. Concentrations of amoebae returned to prior thermal perturbation levels within 30 to 60 days after cessation of thermal additions. Increases in the thermophilic amoeba concentrations were noted in Savannah River oxbows downriver from the Savannah River plant discharge streams as compared with oxbows upriver from the discharges. Concentrations of thermophilic amoebae and thermophilic Naegleria spp. correlated significantly with temperature and conductivity. Air samples taken proximal to the lade during periods of thermal addition showed no evidence of thermophilic Naegleria spp. Isoenzyme patterns of the N. fowleri isolated from the cooling lake were identical to patterns of N. fowleri isolated from other sites in the United States and Belgium.

• DOE Contract Number: AC09-76SR00001
• OSTI ID: 5099015
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:3; ISSN AEMID; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560200* -- Thermal Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMOEBA
ANIMALS
COOLING PONDS
ENVIRONMENTAL EFFECTS
GROWTH
INVERTEBRATES
MICROORGANISMS
NATIONAL ORGANIZATIONS
PATHOGENS
PONDS
PROTOZOA
SARCODINA
SAVANNAH RIVER PLANT
SURFACE WATERS
TEMPERATURE EFFECTS
THERMOPHILIC CONDITIONS
US AEC
US DOE
US ERDA
US ORGANIZATIONS
WATER RESERVOIRS