alpha-Glucosidase-albumin conjugates: effect of chronic administration in mice
Enzyme albumin conjugates have been proposed as a means of increasing the efficacy of enzyme use in vivo and decreasing immune response to the enzyme. Particulate drug carriers, however, have a pronounced tendency to localize in the mononuclear phagocyte (reticuloendothelial) system. The authors have examined in mice the effect on phagocytic index, tissue distribution and organ size of continued administration of conjugates of alpha-glucosidase with either homologous or heterologous albumin. Mice received 10 X 2-mg injections of bovine serum albumin (BSA) or mouse serum albumin (MSA), either free, polymerized or conjugated with alpha-glucosidase. Experiments involving BSA had to be terminated before the end of the experiment because of anaphylaxis, but these reactions were less severe to the polymerized albumin than to free albumin. Free BSA, BSA polymer and BSA-enzyme conjugates all caused a decrease in phagocytic index after six injections. Mice receiving MSA showed no evidence of anaphylaxis, but mice receiving six or more injections of free MSA, MSA polymer or MSA-enzyme conjugate had significantly decreased phagocytic indices as compared to controls. Phagocytic indices had returned to normal by 7 days after the final injection. Tissue distribution of /sup 125/I-labeled albumin preparations was determined in either naive or chronically injected mice.
- Research Organization:
- Univ. of Alberta, Edmonton, Canada
- OSTI ID:
- 5177431
- Journal Information:
- J. Pharmacol. Exp. Ther.; (United States), Journal Name: J. Pharmacol. Exp. Ther.; (United States) Vol. 1; ISSN JPETA
- Country of Publication:
- United States
- Language:
- English
Similar Records
THE EFFECT OF X-IRRADIATION AND PASSIVE ANTIBODY ON IMMUNOLOGIC TOLERANCE IN THE RABBIT TO BOVINE SERUM ALBUMIN
Covalent complexes of albumin with serotonin, ketanserin and lysergic acid antagonize the activity of serotonin in human platelets
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANAPHYLAXIS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLUCOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIPOSOMES
MAMMALS
MICE
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHAGOCYTOSIS
PROTEINS
RADIOISOTOPES
RETICULOENDOTHELIAL SYSTEM
RODENTS
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
VERTEBRATES