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DiO-C/sub 3/-(5) and diS-C/sub 3/-(5): interactions with RBC, ghosts and phospholipid vesicles

Journal Article · · J. Membr. Biol.; (United States)
DOI:https://doi.org/10.1007/BF01870815· OSTI ID:5177166
;

The experiments presented below compare the interaction of diO-C/sub 3/-(5) and diS-C/sub 3/-(5) with erythrocytes, erythrocyte ghosts and phospholipid vesicles derived from erythrocyte membranes. The results confirm earlier reports of diS-C/sub 3/-(5) dimerization in the presence of hemoglobin and of dye aggregate formation in erythrocyte suspensions. DiO-C/sub 3/-(5), on the other hand, binds to vesicles and ghosts freed of hemoglobin in a potential-dependent manner but without forming dye aggregates. The two dyes bind to the different preparations in similar proportions, but diS-C/sub 3/-(5) is bound in amounts 3-40 times greater depending in the degree of polarization. The results show that a mechanism other than binding to hemoglobin must occur in order to explain the potential-dependent binding of both dyes to ghosts and vesicles. A primary interaction must exist between the dye molecule and the lipid bilayer in a biologicl membrane, and this would be expected to occur in the presence of hemoglobin or other cytosolic components. DiO-C/sub 3/-(5) is a better dye to use than diS-C/sub 3/-(5) for mechanistic studies, in order to avoid problems associated with formation of complex aggregates of the latter dye, especially in hyperpolarized membrane suspensions.

Research Organization:
Univ. of Rohester Medical Center, NY
DOE Contract Number:
AC02-76EV03490
OSTI ID:
5177166
Journal Information:
J. Membr. Biol.; (United States), Journal Name: J. Membr. Biol.; (United States) Vol. 59; ISSN JMBBB
Country of Publication:
United States
Language:
English

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Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
AGGLOMERATION
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DIMERIZATION
DISPERSIONS
DYES
ENERGY
ERYTHROCYTES
ESTERS
FLUORESCENCE
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
LIPIDS
LUMINESCENCE
MATERIALS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PIGMENTS
POLARIZATION
POLYMERIZATION
PORPHYRINS
PROTEINS
REACTION KINETICS
SPECTRA
SUSPENSIONS