Short term ethanol alters free radical defense and membrane fatty acid composition in micropigs

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5174496

Zidenberg-Cherr, S; Olin, K L; Villanueva, J; Tang, A; Phinney, S D; Halsted, C H; Keen, C L ^[1]

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1. Univ. of California, Davis (United States)

The authors have suggested that ethanol (Et) metabolism may evoke a protective response by the hepatic cell including changes in free radical defense and membrane fatty acid composition resulting in less peroxidizable membranes. To test this, micropigs were fed diets containing 40% of kcals as either Et or corn starch (C) and 30% of kcals as corn oil. Liver histology was similar in both groups in biopsies taken after 1 mo of treatment. However, liver CuZn superoxide dismutase (CuZnSOD) and glutathione peroxidase activities in Et pigs were 75% of C values, while glutathione reductase and MnSOD activities were 150% of C values. Glutathione levels were higher in Et pigs than in controls. Et pigs had lower TBARS production than C pigs. The peroxidizability index (PI) was calculated from the % of mono-, di-, tri-, penta- and hexaenoic fatty acids and used as an index of substrate availability for lipid peroxidation. Consistent with lower TBARS, PIs in mitochondrial and microsomal fractions were lower in Et pigs than in C pigs. These data show that after short exposure to Et a number of enzymatic and nonenzymatic responses occur in the liver prior to the appearance of histopathology. Whether these changes are part of a compensatory response to Et metabolism or if they contribute to the pathology associated with Et intake needs to be determined.

• OSTI ID: 5174496
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
ETHANOL
GLANDS
GLUTATHIONE
HYDROXY COMPOUNDS
LIVER
MAMMALS
MEMBRANES
METABOLISM
MICROSOMES
MINIATURE SWINE
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES
RIBOSOMES
SWINE
VERTEBRATES