Differential regulation by phorbol ester of formyl-methionyl peptide and leukotriene B sub 4 receptors on human neutrophils

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5172731

Goldman, D W; Chung, S; Richards, S ^[1]

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1. John Hopkins Univ., Baltimore, MD (United States)

Activation of protein kinase C (PKC) with suboptimal does of phorbol myristyl acetate (PMA) will increase fMP receptor expression with parallel potentiation of superoxide generation. PMA-induced changes in leukotriene B{sub 4} (LTB{sub 4}) receptor expression were assessed in parallel with fMP receptor expression to determine if these two independent receptor classes are regulated in a similar manner by PKC. The relative density of fMP receptors was assessed by flow cytometry. The relative density of receptors for LTB{sub 4} was quantitated by incubating 2 {times} 10{sup 6} Ns with 10nM({sup 3}H)-LTB{sub 4} and determining the amount of radioactivity bound after filtration on glass fiber filters. Incubation of N with 10ng/mL PMA induced a 3.2-fold increase in fMP receptor expression by 5 min which was sustained for up to 15 min. In contrast, LTB{sub 4} receptor density decreased by 36% within 5 min. in response to 10 ng/mL PMA. Staurosporine, a potent antagonist of PKC, had no effect of fMP receptor expression but markedly enhanced LTB{sub 4} receptor expression by 1.7-fold at 200nM. PKC acts to decrease the surface expression of LTB{sub 4} receptors in contrast to the enhancement of fMP receptor expression, suggesting in contrast to the enhancement of fMP receptor expression, suggesting that potentiation of N function by PMA may be stimulus-specific.

• OSTI ID: 5172731
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL FLOW SYSTEMS
ENZYME ACTIVITY
ENZYMES
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
NEUTROPHILS
ORGANIC COMPOUNDS
PEPTIDES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
PROSTAGLANDINS
PROTEINS
RECEPTORS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES