Constitutively cellulose-producing mutant of trichoderma reesei

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260240122· OSTI ID:5166033

Mishra, S; Gopalkrishnan, K S; Ghose, T K

The single major factor limiting the economy of enzymatic hydrolysis of cellulose is the cost of the cellulase enzymes. Most of the work to date in reducing this cost has centered around selection of hyperproducing strains using the standard cellulose medium. Cellulose is an insoluble substrate with obvious design disadvantages in bioreactors. It is often indicated that another reasonable approach to the problem would be the isolation of constitutive mutants which require no inducer to form cellulase. In fact, the goal of several laboratories around the world working on the hydrolysis of cellulosic substances has been to isolate a constitutive mutant. Reported here is the isolation of a constitutively cellulase producing mutant in T. reesei that produces high levels of all of the component enzymes in the cellulase complex in the presence of glucose or sucrose. With this constitutive mutant, it is hoped that a major hurdle of working with an insoluble substrate will be overcome. The mutant will allow easier operation and control of cellulose bioreactors and can be used for continuous enzyme production, as opposed to the present batch production system. Also, a number of other unusable susbtrates like whey and molasses can be used for commercial preparations of cellulases. (Refs. 2).

• Research Organization: Biochemical Engineering Research Centre, Indian Inst of Tech, Delhi, Hauz Khas, New Delhi-110016, India
• OSTI ID: 5166033
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24:1; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOHYDRATES
CELLULASE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MUTAGEN SCREENING
MUTANTS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PRODUCTION
SACCHARIDES
SCREENING
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
TRICHODERMA VIRIDE
YIELDS