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Title: Cannabinoids induce incomplete maturation of cultured human leukemia cells

Abstract

Monocyte maturation markers were induced in cultured human myeloblastic ML-2 leukemia cells after treatment for 1-6 days with 0.03-30 ..mu..M ..delta../sup 9/-tetrahydrocannabinol (THC), the major psychoactive component of marijuana. After a 2-day or longer treatment, 2- to 5-fold increases were found in the percentages of cells exhibiting reactivity with either the murine OKM1 monoclonal antibody of the Leu-M5 monoclonal antibody, staining positively for nonspecific esterase activity, and displaying a promonocyte morphology. The increases in these differentiation markers after treatment with 0.03-1 ..mu..M THC were dose dependent. At this dose range, THC did not cause an inhibition of cell growth. The THC-induced cell maturation was also characterized by specific changes in the patterns of newly synthesized proteins. The THC-induced differentiation did not, however, result in cells with a highly developed mature monocyte phenotype. However, treatment of these incompletely matured cells with either phorbol 12-myristate 13-acetate of 1..cap alpha..,25-dihydroxycholecalciferol, which are inducers of differentiation in myeloid leukemia cells (including ML-2 cells), produced cells with a mature monocyte morphology. The ML-2 cell system described here may be a useful tool for deciphering critical biochemical events that lead to the cannabinoid-induced incomplete cell differentiation of ML-2 cells and other related cell types. Findings obtainedmore » from this system may have important implications for studies of cannabinoid effects on normal human bone-marrow progenitor cells.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab., IL (USA)
OSTI Identifier:
5164483
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:15
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MARIHUANA; BIOLOGICAL EFFECTS; TUMOR CELLS; AUTORADIOGRAPHY; CELL DIFFERENTIATION; BIOLOGICAL MARKERS; CARCINOGENESIS; DOSE-RESPONSE RELATIONSHIPS; HALLUCINOGENS; LEUKEMIA; MAN; METHIONINE; MONOCLONAL ANTIBODIES; MONOCYTES; PHORBOL ESTERS; SULFUR 35; TWO-DIMENSIONAL ELECTROPHORESIS; AMINO ACIDS; ANIMAL CELLS; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CARCINOGENS; CENTRAL NERVOUS SYSTEM AGENTS; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELECTROPHORESIS; ESTERS; EVEN-ODD NUCLEI; HEMIC DISEASES; HERBS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; MATERIALS; NEOPLASMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PATHOGENESIS; PLANTS; PRIMATES; PSYCHOTROPIC DRUGS; RADIOISOTOPES; SULFUR ISOTOPES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Murison, G, Chubb, C B.H., Maeda, S, Gemmell, M A, and Huberman, E. Cannabinoids induce incomplete maturation of cultured human leukemia cells. United States: N. p., 1987. Web. doi:10.1073/pnas.84.15.5414.
Murison, G, Chubb, C B.H., Maeda, S, Gemmell, M A, & Huberman, E. Cannabinoids induce incomplete maturation of cultured human leukemia cells. United States. https://doi.org/10.1073/pnas.84.15.5414
Murison, G, Chubb, C B.H., Maeda, S, Gemmell, M A, and Huberman, E. Sat . "Cannabinoids induce incomplete maturation of cultured human leukemia cells". United States. https://doi.org/10.1073/pnas.84.15.5414.
@article{osti_5164483,
title = {Cannabinoids induce incomplete maturation of cultured human leukemia cells},
author = {Murison, G and Chubb, C B.H. and Maeda, S and Gemmell, M A and Huberman, E},
abstractNote = {Monocyte maturation markers were induced in cultured human myeloblastic ML-2 leukemia cells after treatment for 1-6 days with 0.03-30 ..mu..M ..delta../sup 9/-tetrahydrocannabinol (THC), the major psychoactive component of marijuana. After a 2-day or longer treatment, 2- to 5-fold increases were found in the percentages of cells exhibiting reactivity with either the murine OKM1 monoclonal antibody of the Leu-M5 monoclonal antibody, staining positively for nonspecific esterase activity, and displaying a promonocyte morphology. The increases in these differentiation markers after treatment with 0.03-1 ..mu..M THC were dose dependent. At this dose range, THC did not cause an inhibition of cell growth. The THC-induced cell maturation was also characterized by specific changes in the patterns of newly synthesized proteins. The THC-induced differentiation did not, however, result in cells with a highly developed mature monocyte phenotype. However, treatment of these incompletely matured cells with either phorbol 12-myristate 13-acetate of 1..cap alpha..,25-dihydroxycholecalciferol, which are inducers of differentiation in myeloid leukemia cells (including ML-2 cells), produced cells with a mature monocyte morphology. The ML-2 cell system described here may be a useful tool for deciphering critical biochemical events that lead to the cannabinoid-induced incomplete cell differentiation of ML-2 cells and other related cell types. Findings obtained from this system may have important implications for studies of cannabinoid effects on normal human bone-marrow progenitor cells.},
doi = {10.1073/pnas.84.15.5414},
url = {https://www.osti.gov/biblio/5164483}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:15,
place = {United States},
year = {1987},
month = {8}
}