Does atrial natriuretic factor protect against right ventricular overload II. Tissue binding

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · Journal of Applied Physiology (1985); (USA)

OSTI ID:5161667

Ou, L C; Yen, S; Sardella, G L; Hill, N S ^[1]

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1. Dartmouth Medical School, Hanover, NH (USA)

Previous studies have led us to hypothesize that the physiological significance of the diuretic and pulmonary vaso-relaxant effects of atrial natriuretic factor (ANF) is to protect the right heart. This study was designed to evaluate the relative importance of various peripheral tissues as sites of ANF action by tracing the temporal pattern of distribution of {sup 125}I-ANF and quantitating the specific binding sites. An in vivo approach, utilizing trace amount of {sup 125}I-ANF was adopted to simulate physiological conditions. {sup 125}I-ANF injected either intravenously or intra-arterially was quickly bound to peripheral tissues with less than 5% remaining in the circulation after 1 min. The relative binding capacity was greatest in the lung, followed by the kidney, right ventricle, adrenal gland, and left ventricle. The magnitude of specific ANF binding sites per gram of tissue weight followed a similar order. The data demonstrate that ANF released under all circumstances is quickly bound to the target organs, particularly the lung and the kidney, and suggest that these two organs could be the most important target organs of ANF. This evidence provides further support for the proposed hypothesis that a major evolutionary role of ANF is the protection of the right ventricle from mechanical loads.

• OSTI ID: 5161667
• Journal Information: Journal of Applied Physiology (1985); (USA), Journal Name: Journal of Applied Physiology (1985); (USA) Vol. 67:4; ISSN JAPHE; ISSN 8750-7587
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
FUNCTIONS
GLANDS
HEART
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LUNGS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES