Neutron irradiation of human melanoma cells

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Pigment Cell Research; (USA)

DOI:https://doi.org/10.1111/j.1600-0749.1989.tb00211.x· OSTI ID:5157656

Brown, K; Mountford, M H; Allen, B J; Mishima, Y; Ichihashi, M; Parsons, P ^[1]

---

1. Australian Nuclear Science and Technology Organisation, Lucas Heights Research Laboratory, Menai, New South Wales

The biological characteristics and in vitro radiosensitivity of melanoma cells to thermal neutrons were investigated as a guide to the effectiveness of boron neutron capture therapy. Plateau phase cultures of three human malignant melanoma-established cell lines were examined for cell density at confluence, doubling time, cell cycle parameters, chromosome constitution, and melanin content. Cell survival dose-response curves, for cells preincubated in the presence or absence of p-boronophenylalanine. HCl (10B1-BPA), were measured over the dose range 0.6-8.0 Gy (N + gamma). The neutron fluence rate was 2.6 x 10(9) n/cm2/s and the total dose rate 3.7 Gy/h (31% gamma). Considerable differences were observed in the morphology and cellular properties of the cell lines. Two cell lines (96E and 96L) were amelanotic, and one was melanotic (418). An enhanced killing for neutron irradiation was found only for the melanotic cells after 20 h preincubation with 10 micrograms/ml 10B1-BPA. In view of the doubling times of the cell lines of about 23 h (96E and 96L) or of 36 h (418), it seems likely that an increased boron uptake, and hence increased radiosensitivity, might result if the preincubation period with 10B1-BPA is extended to several hours longer than the respective cell cycle times.

• OSTI ID: 5157656
• Journal Information: Pigment Cell Research; (USA), Journal Name: Pigment Cell Research; (USA) Vol. 2:4; ISSN 0893-5785; ISSN PCREE
• Country of Publication: United States
• Language: English

Related Subjects

550603* -- Medicine-- External Radiation in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BORON COMPOUNDS
CARBOXYLIC ACIDS
CELL CYCLE
DISEASES
DOSE-RESPONSE RELATIONSHIPS
MAMMALS
MAN
MEDICINE
MELANOMAS
NEOPLASMS
NEUTRON CAPTURE THERAPY
NEUTRON THERAPY
NUCLEAR MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PRIMATES
RADIOLOGY
RADIOSENSITIVITY
RADIOTHERAPY
RBE
THERAPY
TUMOR CELLS
VERTEBRATES