Rhesus monkey lens as an in vitro model for studying oxidative stress

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · Investigative Ophthalmology amp Visual Science; (USA)

OSTI ID:5153433

Zigler, Jr, J S; Lucas, V A; Du, X Y ^[1]

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1. National Eye Institute, Bethesda, MD (USA)

Lenses from young rhesus monkeys were incubated in the presence of H{sub 2}O{sub 2} or oxygen radical generating systems to determine their suitability as a model for investigating lenticular oxidative stress. Additionally, direct comparisons were made between the effects found with the monkey lenses and those observed with cultured rat lenses exposed to the same oxidizing systems. As in earlier studies with rat lenses the monkey lenses exhibited impaired ability to actively accumulate from the medium radioactively labelled rubidium and choline following exposure to oxidative stress. Based on the effects of various scavengers of oxygen radicals it appeared that the mechanisms responsible for lens damage were the same for both rat and monkey lenses. However, rat lenses were damaged by lower concentrations of oxidants than were monkey lenses. It was concluded that oxidative stress affects both rat and monkey lenses by similar mechanisms but that lenses from monkeys, and probably other primates, are more resistant to these effects because they have better endogenous antioxidant defenses.

• OSTI ID: 5153433
• Journal Information: Investigative Ophthalmology amp Visual Science; (USA), Vol. 30:10; ISSN 0146-0404
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CRYSTALLINE LENS
BIOLOGICAL STRESS
HYDROGEN PEROXIDE
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
CHOLINE
CULTURE MEDIA
GENETIC VARIABILITY
MONKEYS
OXYGEN
RADICALS
RADIOISOTOPES
RATS
RUBIDIUM
TRACER TECHNIQUES
ALCOHOLS
ALKALI METALS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOLOGICAL VARIABILITY
BODY
BODY AREAS
DRUGS
ELEMENTS
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
METALS
NONMETALS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEROXIDES
PRIMATES
QUATERNARY COMPOUNDS
RODENTS
SENSE ORGANS
VERTEBRATES
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