Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(85)90292-1· OSTI ID:5151554

Rosenthal, G A; Thomas, D A

Procedures for a radiometric assay of L-(guanidinooxy-/sup 14/C)canavanine were developed which provide a convenient and accurate measure of the incorporation of (/sup 14/C)canavanine into de novo-synthesized proteins. These methods are also applicable to determining (/sup 14/C)arginine incorporation into protein. These procedures have been employed to study the synthesis of L-(guanidinooxy-/sup 14/C)canavanine- and L-(guanidino-/sup 14/C)arginine-containing proteins from the hemolymph of Manduca sexta and Heliothis virescens, two highly destructive insect pests.

• Research Organization: Univ. of Kentucky, Lexington
• OSTI ID: 5151554
• Journal Information: Anal. Biochem.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
ARGININE
CARBON 14 COMPOUNDS
INSECTS
RADIOASSAY
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
ARTHROPODS
CARBOXYLIC ACIDS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques