Radiolabeling of a wound-inducible pyridoxal phosphate utilizing protein from tomato: evidence for its identification as ACC synthase
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5151295
Aminocyclopropane 1-carboxylic acid (ACC) synthase, a pyridoxal phosphate utilizing enzyme, catalyzes the conversion of S-adenosylmethionine to ACC, the rate limiting step in the biosynthesis of the plant hormone, ethylene. Ethylene, besides being involved in normal plant growth processes, is also produced in response to stress, e.g. wounding, pathogen infection, etc. The authors report the partial purification (400 fold) of ACC synthase from wounded pink tomato pericarp by classical techniques including ammonium sulfate precipitation, ion exchange and phenyl sepharose chromatography. Further purification results in a decrease in specific activity apparently due to the instability of the enzyme and the low levels present in plant tissue. Radiolabeling of a pyridoxal phosphate-utilizing protein in the ACC synthase enriched fraction was achieved. Evidence that this radiolabeled protein is ACC synthase will be presented. Amino acid sequence determination of putative ACC synthase-derived peptides is underway.
- Research Organization:
- CIBA-GEIGY, Research Triangle Park, NC
- OSTI ID:
- 5151295
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Conference
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Tue May 01 00:00:00 EDT 1990
· Plant Physiology, Supplement; (USA)
·
OSTI ID:5629233
Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits
Journal Article
·
Sat Jun 15 00:00:00 EDT 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:6100411
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Journal Article
·
Mon Oct 01 00:00:00 EDT 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5584167
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKENES
AMINO ACID SEQUENCE
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
ETHYLENE
FOOD
FRUITS
GROWTH
HYDROCARBONS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGASES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANT GROWTH
PROTEINS
PURIFICATION
RADIOISOTOPES
SEPARATION PROCESSES
SYNTHESIS
TOMATOES
TRACER TECHNIQUES
WOUNDS
59 BASIC BIOLOGICAL SCIENCES
ALKENES
AMINO ACID SEQUENCE
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
ETHYLENE
FOOD
FRUITS
GROWTH
HYDROCARBONS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGASES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANT GROWTH
PROTEINS
PURIFICATION
RADIOISOTOPES
SEPARATION PROCESSES
SYNTHESIS
TOMATOES
TRACER TECHNIQUES
WOUNDS