Radiolabeling of a wound-inducible pyridoxal phosphate utilizing protein from tomato: evidence for its identification as ACC synthase

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5151295

Privalle, L S; Graham, J S; Caughey, P A

Aminocyclopropane 1-carboxylic acid (ACC) synthase, a pyridoxal phosphate utilizing enzyme, catalyzes the conversion of S-adenosylmethionine to ACC, the rate limiting step in the biosynthesis of the plant hormone, ethylene. Ethylene, besides being involved in normal plant growth processes, is also produced in response to stress, e.g. wounding, pathogen infection, etc. The authors report the partial purification (400 fold) of ACC synthase from wounded pink tomato pericarp by classical techniques including ammonium sulfate precipitation, ion exchange and phenyl sepharose chromatography. Further purification results in a decrease in specific activity apparently due to the instability of the enzyme and the low levels present in plant tissue. Radiolabeling of a pyridoxal phosphate-utilizing protein in the ACC synthase enriched fraction was achieved. Evidence that this radiolabeled protein is ACC synthase will be presented. Amino acid sequence determination of putative ACC synthase-derived peptides is underway.

• Research Organization: CIBA-GEIGY, Research Triangle Park, NC
• OSTI ID: 5151295
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ETHYLENE
BIOSYNTHESIS
LIGASES
ENZYME ACTIVITY
PURIFICATION
PEPTIDES
AMINO ACID SEQUENCE
PROTEINS
LABELLING
CARBOXYLIC ACIDS
CHROMATOGRAPHY
PHOSPHATES
PLANT GROWTH
RADIOISOTOPES
TOMATOES
TRACER TECHNIQUES
WOUNDS
ALKENES
ENZYMES
FOOD
FRUITS
GROWTH
HYDROCARBONS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques