Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits
- Michigan State Univ., East Lansing (United States)
- Berlex Biosciences, Alameda, CA (United States)
1-Aminocyclopropane-1-carboxylate synthase is the regulated enzyme in the biosynthetic pathway of the plant hormone ethylene. A full-length cDNA encoding this enzyme has been cloned from tomato fruits. The authors report here the complete nucleotide and derived amino acid sequences of a cDNA encoding a second isoform of ACC synthase from tomato fruits. The cDNAs coding for both isoforms contain highly conserved regions that are surrounded by regions of low homology, especially at the 5{prime} and 3{prime} ends. Gene-specific probes were constructed to examine the expression of transcripts encoding the two ACC synthase isoforms under two conditions of enhanced ethylene formation--namely, during fruit ripening and in response to mechanical stress (wounding). The level of mRNA encoding both isoforms, ACC synthase 1 and 2, increased during ripening. In contrast, wounding caused an increase in only the level of mRNA coding for ACC synthase 1. Blot analysis of genomic DNA digested with restriction enzymes confirmed that ACC synthase 1 and 2 are encoded by different genes.
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 6100411
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:12; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYASES
GENE REGULATION
RECOMBINANT DNA
DNA SEQUENCING
AMINO ACID SEQUENCE
DNA HYBRIDIZATION
MESSENGER-RNA
PHOSPHORUS 32
TOMATOES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
FOOD
FRUITS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques