Tyrosine aminotransferase mRNA turnover in CHO cells
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5148380
- Oak Ridge National Lab., TN (United States) Univ. of Tennessee, Oak Ridge (United States)
An expression vector containing a minigene designed for precise expression of the mRNA and the protein of rat tyrosine aminotransferase (TAT), ordinarily only expressed in liver, was constructed. This minigene was placed under control of the LTR promoter of murine leukemia virus and was cotransfected into CHO cells deficient in dihydrofolate reductase (DHFR) with another vector (pSV2dhfr) encoding DHFR. Upon methotrexate selection and further amplification, cell lines which produce large amounts of TAT mRNA and the enzyme have been isolated. Northern blots exhibited the expected 2.4 kb band of TAT mRNA, and S1-nuclease protection assay established that the mRNA was correctly initiated and terminated. When transcription was blocked with actinomycin, the 2.4 kb TAT mRNA in CHO cell lines was degraded with half-life ranging 1.5 to 2.5 hr, a rate similar to that found in liver or hepatoma cells. Furthermore, the rapid turnover of TAT mRNA in CHO cells could be completely stabilized by cyclohexamide as observed previously in rat liver. With their high content of TAT mRNA exhibiting normal turnover properties, the CHO cell lines established should be useful for analysis of the molecular mechanisms of intracellular turnover of this labile message.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5148380
- Report Number(s):
- CONF-9104107--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of cAMP on the stability of tyrosine aminotransferase mRNA
Isolation of a functional, insulin regulatable glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene
Insulin enhances transcription of the tyrosine aminotransferase gene in rat liver
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6359344
Isolation of a functional, insulin regulatable glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6360815
Insulin enhances transcription of the tyrosine aminotransferase gene in rat liver
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5234618
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
AMINO ACIDS
AMINOTRANSFERASES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
CARBOXYLIC ACIDS
CHO CELLS
DISEASES
DRUGS
ENZYMES
GENE AMPLIFICATION
GENE REGULATION
GENES
HYDROXY ACIDS
IMMUNE SYSTEM DISEASES
LEUKEMIA
MAMMALS
MESSENGER-RNA
METABOLISM
MICE
NEOPLASMS
NITROGEN TRANSFERASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RNA
RODENTS
TRANSCRIPTION
TRANSFERASES
TYROSINE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
AMINO ACIDS
AMINOTRANSFERASES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
CARBOXYLIC ACIDS
CHO CELLS
DISEASES
DRUGS
ENZYMES
GENE AMPLIFICATION
GENE REGULATION
GENES
HYDROXY ACIDS
IMMUNE SYSTEM DISEASES
LEUKEMIA
MAMMALS
MESSENGER-RNA
METABOLISM
MICE
NEOPLASMS
NITROGEN TRANSFERASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RNA
RODENTS
TRANSCRIPTION
TRANSFERASES
TYROSINE
VERTEBRATES