Comparison of beta-glucosidase activities in different Streptomyces strains. [S flavogriseus and S. strain CB-12]

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5147453

Moldoveanu, N; Kluepfel, D

Cellobiase (beta-glucosidase) production was compared for two streptomycetes: Streptomyces flavogriseus, a known producer of cellulase complex, and Streptomyces species strain CB-12, a strain isolated for its rapid growth on cellobiose. The optimal conditions for enzyme activity were established in relation to pH, temperature, enzyme stability, and substrate affinity. The production of beta-glucosidase by the two strains depended on the carbon substrate in the medium. Cellobiose was found to repress the biosynthesis of the enzyme in S. flavogriseus and to stimulate its production in strain CB-12. The biosynthesis of the enzyme correlated well with the accumulation of glucose in the culture filtrates. The combined action of the beta-glucosidases produced by the two Streptomyces strains might allow a better utilization of the reaction products which arise during the biodegradation of cellulose.

• Research Organization: Centre de recherche en bacteriologie, Institut Armand-Frappier, Univ. du Quebec, Laval, Quebec H7N 4Z3, Canada
• OSTI ID: 5147453
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 46:1; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIODEGRADATION
BIOSYNTHESIS
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CULTURE MEDIA
DECOMPOSITION
DISACCHARIDES
ENZYME ACTIVITY
ENZYMES
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
INHIBITION
MICROORGANISMS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
SACCHARIDES
STABILITY
STREPTOMYCES
SUBSTRATES
SYNTHESIS
TEMPERATURE DEPENDENCE