axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase

Journal Article · Tue Oct 01 04:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.10.5016· OSTI ID:5144898

O'Bryan, J P; Frye, R A; Cogswell, P C; Neubauer, A; Kitch, B; Prokop, C; Earp, H S; Liu, E T ^[1]; Espinosa, R III; Le Beau, M M ^[2]

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1. Univ. of North Carolina, Chapel Hill (United States)
2. Univ. of Chicago, IL (United States)

Using a sensitive transfection-tumorigenicity assay, the authors have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein. Expression of axl cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antophosphotyrosine antibodies, confirming that the axl protein is a tyrosine kinase. The juxtaposition of fibronectin type II and immunoglobulinlike repeats in the extracellular domain, as well as distinct amino acid sequences in the kinase domain, indicate that the axl protein represents a novel subclass of receptor tyrosine kinases.

• OSTI ID: 5144898
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:10; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
COAGULANTS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA SEQUENCING
DRUGS
ENZYMES
FIBROBLASTS
GENE REGULATION
GENES
HEMATOLOGIC AGENTS
HYDROXY ACIDS
IMMUNE SYSTEM DISEASES
LEUKEMIA
MEMBRANE PROTEINS
MYELOID LEUKEMIA
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RECEPTORS
RECOMBINANT DNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
TUMOR CELLS
TYROSINE