Assignment of the human GAS6 gene to chromosome 13q34 by fluorescene in situ hybridization

Journal Article · Wed Nov 01 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.0027· OSTI ID:273474

Saccone, S; Della Valle, G ^[1]; Marcandalli, P; Gostissa, M; Schneider, C ^[2]

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1. Universita di Pavia (Italy)
2. Laboratorio Nazionale Consorzio Interuniversitario per le Biotecnologie, Trieste (Italy); and others

GAS6 was originally isolated as a set of growth-arrest-specific (gas) genes whose expression is increased at growth arrest and becomes negatively regulated after synchronous cell cycle induction in murine NIH3T3 cells. Sequence of the human full-length cDNA homologue revealed that the protein encoded belongs to the class of vitamin K-dependent proteins, more specifically related to protein S, a negative coregulator in the blood coagulation cascade (43% aa identity). The regions with the highest degree of similarity between Gas6 and the related protein S comprise the Gla domain ({gamma} carboxylated glutamic acid-rich region) and the four EFG-like domains. In between these two regions, protein S presents a linking segment with a thrombin-sensitive site, which has been lost by Gas6. Recently, it has been demonstrated that Gas6 is the ligand of the tyrosine kinase receptor Axl. Axl is the first described member of a receptor tyrosine kinase family comprising various members that have in common the presence of tandem repeats of immunoglobulin-like and fibronectin type III domains in the extracellular portion of the receptor. In this report we describe the chromosomal localization of GAS6 using fluorescence in situ hybridization on metaphase chromosomes. 10 refs., 1 fig.

• OSTI ID: 273474
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 30; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACIDS
DNA HYBRIDIZATION
DNA SEQUENCING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 13
LIGANDS
PHOSPHOTRANSFERASES
RECEPTORS
TYROSINE