No effect of 5-fluorouracil on the properties of purified. cap alpha. -amylase from barley half-seeds
Amylase has been purified from de-embryonated seeds of barley (Horedeum vulgare L. cv. Betzes) which have been incubated on 10/sup 6/M gibberellic acid (GA/sub 3/) following 3 days of imbibition in buffer. Incubation of the half-seeds in up to 10/sup -2/ M 5-fluorouracil (5-FU) during the entire incubation period, including imbibition, had no effect on any of the following characteristics of purified ..cap alpha..-amylase: thermal stability in the absence of calcium, molecular weight of the enzyme, isozyme composition, specific activity, or the amount of ..cap alpha..-amylase synthesized by the aleurone tissue. The synthesis of rRNA and tRNA was strongly inhibited by 5-FU, indicating that the analog had entered the aleurone cells. These results are not in agreement with those of Carlson (Nature New Biology 237: 39-41 (1972)) who found that treatment of barley aleurone with 10/sup -4/ M 5-FU to the addition of GA/sub 3/ resulted in decreased thermal stability of GA/sub 3/-induced ..cap alpha..-amylase and who interpreted this as evidence that the mRNA for ..cap alpha..-amylase was synthesized during the imbibition of the aleurone tissue and independently of gibberellin action. Results of the present experiments indicate that the thermal stability of highly purified ..cap alpha..-amylase is not altered by treatment of barley half-seeds with 5-FU, and that 5-FU cannot be used as a probe to examine the timing of ..cap alpha..-amylase mRNA synthesis.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5143529
- Journal Information:
- Plant Physiol.; (United States), Vol. 61:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMYLASE
BIOSYNTHESIS
RNA
BARLEY
BIOLOGICAL EFFECTS
CALCIUM
ENZYMES
FLUOROURACILS
SEEDS
ALKALINE EARTH METALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOMASS
CEREALS
DRUGS
ELEMENTS
ENERGY SOURCES
GLYCOSYL HYDROLASES
GRAMINEAE
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
METALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRIMIDINES
RENEWABLE ENERGY SOURCES
SYNTHESIS
URACILS
550200* - Biochemistry