The Saccharomyces cerevisiae ACP2 gene encodes an essential HMG1-like protein

Journal Article · Tue Mar 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.3.1282· OSTI ID:5132353

Haggren, W; Kolodrubetz, D

The high-mobility-group (HMG) proteins, a group of nonhistone chromatin-associated proteins, have been extensively characterized in higher eucaryotic cells. To test the biological function of an HMG protein, the authors cloned and mutagenized a gene encoding an HMG-like protein from the yeast Saccharomyces cerevisiae. A yeast genomic DNA library was screened with an oligonucleotide designed to hybridize to any yeast gene containing an amino acid sequence conserved in several higher eucaryotic HMG proteins. DNA sequencing and Northern (RNA) blot analysis revealed that one gene, called ACP2 (acidic protein 2), synthesizes a poly(A)/sup +/ RNA in S. cerevisiae which encodes a 27,000-molecular-weight protein whose amino acid sequence is homologous to those of calf HMG1 and trout HMGT proteins. Standard procedures were used to construct a diploid yeast strain in which one copy of the ACP2 gene was mutated by replacement with the URA3 gene. When this diploid was sporulated and dissected, only half of the spores were viable. About half of the nonviable spores proceeded through two or three cell divisions and then stopped dividing; the rest did not germinate at all. None of the viable spores contained the mutant ACP2 gene, thus proving that the protein encoded by ACP2 is required for cell viability. The results presented here demonstrate that an HMG-like protein has an essential physiological function.

• Research Organization: Dept. of Microbiology, The Univ. of Texas Health Science Center at San Antonio, San Antonio, TX (US)
• OSTI ID: 5132353
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:3; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BIOLOGICAL FUNCTIONS
CLONING
DIPLOIDY
DNA-CLONING
FUNCTIONS
FUNGI
GENE RECOMBINATION PROTEINS
GENETIC MAPPING
HYBRIDIZATION
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
MUTANTS
NUCLEIC ACIDS
NUCLEOPROTEINS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHYSIOLOGY
PLANTS
PLOIDY
PROTEINS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
YEASTS