Alternative processing of mRNAs encoding mammalian chromosomal high-mobility-group proteins HMG-I and HMG-Y

Journal Article · Mon May 01 04:00:00 EDT 1989 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.9.5.2114· OSTI ID:5604354

Johnson, K R; Reeves, R; Lehn, D A

The high-mobility-group protein HMG-I is a well-characterized nonhistone chromosomal protein that is preferentially expressed in rapidly dividing cells, binds to A . T-rich regions of DNA in vitro, and has been localized to particular regions of mammalian metaphase chromosomes. The authors isolated eight cDNA clones encoding HMG-I and its isoform HMG-Y from a human Raji cell cDNA library and detected blocks of nucleotide sequence rearrangements in the 5'-untranslated regions of these clones. In addition to this leader sequence variation, five of the eight cDNA clones had either a 33- or 3-base-pair in-frame deletion in their open reading frame (ORF); the author found that this shortened ORF encodes the HMG-Y protein isoform. They present evidence that the 5'-untranslated-region and ORF heterogeneity of the cDNA clones is the result of alternative processing of RNA transcripts from a single functional gene. Several additional but probably nonfunctional HMG-1 or HMG-Y gene copies exist in the human genome; they isolated and partially sequenced one of these pseudogenes and found that is a processed HMG-Y retropseudogene.

• Research Organization: Washington State Univ., Pullman, WA (USA). Dept. of Biochemistry and Biophysics
• OSTI ID: 5604354
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 9:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL FUNCTIONS
CELL DIVISION
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FUNCTIONS
GENE OPERONS
GENE REGULATION
GROWTH FACTORS
HYBRIDIZATION
IN VITRO
MESSENGER-RNA
MITOGENS
MITOSIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHENOTYPE
PROTEINS
RNA
RNA PROCESSING
STRUCTURAL CHEMICAL ANALYSIS