GC/MS confirmatory method for etorphine in horse urine

Journal Article · · Journal of Analytical Toxicology; (USA)

DOI:https://doi.org/10.1093/jat/13.4.193· OSTI ID:5131923

Bonnaire, Y; Plou, P ^[1]; Pages, N; Boudene, C ^[2]; Jouany, J M ^[3]

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1. Laboratoire de la Federation Nationale des Societes de Courses, Chatenay-Malabry (France)
2. Universite de Paris XI (France)
3. Universite de Rouen (France)

A highly sensitive procedure for GC/MS determine of etorphine in horse urine is described. This assay provides both specificity and reliability and is particularly well suited for the confirmation of radioimmunoassay screening procedures usually used for etorphine. After solvent extraction and purifications, the etorphine is characterized as a pentafluoroacetic derivative (PFAA) by using mass fragmentography. The detection limit is O.1 ng/mLin urine; the coefficient of variation of the estimations is 10.9%. The procedure has been validated after on-field administration of 5 to 90 {mu}g of etorphine to five thoroughbred horses of 5 to 90 {mu}g of etorphine to five thoroughbred horses (10 to 180 ng/kg).

• OSTI ID: 5131923
• Journal Information: Journal of Analytical Toxicology; (USA), Journal Name: Journal of Analytical Toxicology; (USA) Vol. 13:4; ISSN 0146-4760; ISSN JATOD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACCURACY
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIAGNOSTIC TECHNIQUES
DRUGS
HORSES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
NARCOTICS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SENSITIVITY
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES