Nearest neighbor affects G:C to A:T transitions induced by alkylating agents
The influence of local DNA sequence on the distribution of G:C to A:T transitions induced in the lacI gene of E. coli by a series of alkylating agents has been analyzed. In the case of nitrosoguanidine, two nitrosoureas and a nitrosamine, a strong preference for mutation at sites proceeded 5' by a purine base was noted. This preferences was observed with both methyl and ethyl donors where the predicted common ultimate alkylating species in the alkyl diazonium ion. In contrast, this preferences was not seen following treatment with ethylmethanesulfonate. The observed preference for 5'PuG-3' site over 5'-PyG-3' sites corresponds well with alterations observed in the Ha-ras oncogene recovered after treatment with NMU. This indicates that the mutations recovered in the oncogenes are likely the direct consequence of the alkylation treatment and that the local sequence effects seen in E. coli also appear to occur in mammalian cells.
- Research Organization:
- York Univ., Toronto, Ontario (Canada)
- OSTI ID:
- 5129775
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 76; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mutational specificities of environmental carcinogens in the lacl gene of Escherichia coli H. V: DNA sequence analysis of mutations in bacteria recovered from the liver of Swiss mice exposed to 1,2-dimethylhydrazine, azoxymethane, and methylazoxymethanolacetate
DNA sequence analysis of mutagenicity and site specificity of ethyl methanesulfonate in Uvr+ and UvrB- strains of Escherichia coli
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ADENINES
ALKYLATING AGENTS
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BACTERIA
BIOLOGICAL EFFECTS
CARCINOGENESIS
CYTOSINE
DNA ADDUCTS
DRUGS
ESCHERICHIA COLI
GENE MUTATIONS
GENES
GENETIC EFFECTS
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
MUTATIONS
NITROSO COMPOUNDS
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PATHOGENESIS
PURINES
PYRIMIDINES