Function of plastid mRNA 3' inverted repeats. RNA stabilization and gene-specific protein binding

Journal Article · Sun Nov 05 00:00:00 EST 1989 · Journal of Biological Chemistry; (USA)

OSTI ID:5129458

Stern, D B; Jones, H; Gruissem, W ^[1]

---

1. Univ. of California, Berkeley (USA)

Plastid protein coding regions in plants are generally flanked by 3' inverted repeat (IR) sequences. In a previous work, we have shown that their role may be in RNA stabilization and as a processing signal that establishes the mature mRNA 3' end. In this report we have investigated the stability and protein interaction of chloroplast mRNA 3' IR-RNA sequences in more detail. Progressive deletions into the 3' IR-RNA sequences for the chloroplast cytochrome b6/f subunit IV (petD) mRNA reduce the stability of the RNA, indicating that the potential to form a stem/loop is a minimum requirement for petD 3' IR-RNA stability in vitro. Specific point mutants also destabilize the processed 3' IR-RNA, suggesting an important role for the primary sequence. Gel mobility shift and UV-cross-linking analysis has shown that 3' IR-RNAs of petD and two other chloroplast mRNAs (rbcL and psbA) interact with proteins in vitro. Comparison of the bound petD 3' IR-RNA proteins with proteins that bind to rbcL and psbA reveals that binding of certain proteins is gene-specific. Also, precursor and processed petD 3' IR-RNAs bind different sets of proteins. A single nucleotide transversion (T----A) near the base of the stem eliminates the binding of a 29-kDa protein to the petD 3' IR-RNA precursor. We discuss the possible role of 3' IR-RNA-protein interactions in plastid mRNA 3' end maturation and differential mRNA stability.

• OSTI ID: 5129458
• Journal Information: Journal of Biological Chemistry; (USA), Vol. 264:31; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MESSENGER-RNA
STABILITY
PROTEINS
GENES
RNA
BIOLOGICAL FUNCTIONS
AMINO ACID SEQUENCE
CHLOROPLASTS
CHROMATOGRAPHY
CPB
CROSS-LINKING
CYTOCHROMES
MUTATIONS
PLANTS
PRECURSOR
ULTRAVIOLET RADIATION
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
FUNCTIONS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
POLYMERIZATION
RADIATIONS
SEPARATION PROCESSES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture