Differential regulation of plastid mRNA stability
Technical Report
·
OSTI ID:7263195
Our view of MRNA decay is that it is regulated by three interactive components: RNA structure, ribonucleases and RNAbinding proteins. We have used mutagenesis to study the role of RNA structure in regulating RNA decay rates, and to identify protein binding and endonuclease recognition sites. We have identified at least three endonuclease activities; one that cleaves psbA RNA; and two whose cleavage patterns with penD 3{prime}IR-RNA has been studied (endoC1 and endoC2). Additionally, we have continued to analyze the properties of the major RNA processing exoribonuclease. We have concentrated our efforts on three RNA-binding proteins. A 100 kd protein with properties suggestive of a mammalian RNP component has been purified. A protein of 55 kd that may also be an endonuclease has been partially purified. We have studied the interaction of a 29 kd protein with the pend stem/loop, and its role in RNA processing. Recently, we have used a novel gel shift/SDS-PAGE technique to identify new RNA-binding proteins that form complexes of as-yet unknown function.
- Research Organization:
- Boyce Thompson Inst. for Plant Research, Ithaca, NY (United States)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER20015
- OSTI ID:
- 7263195
- Report Number(s):
- DOE/ER/20015-1; ON: DE92040451
- Country of Publication:
- United States
- Language:
- English
Similar Records
Differential regulation of plastid mRNA stability. Progress report
Differential regulation of plastid mRNA stability. Progress report
An AU-rich element in the 3{prime} untranslated region of the spinach chloroplast petD gene participates in sequence-specific RNA-protein complex formation
Technical Report
·
Tue Sep 01 00:00:00 EDT 1992
·
OSTI ID:10176585
Differential regulation of plastid mRNA stability. Progress report
Technical Report
·
Wed Sep 01 00:00:00 EDT 1993
·
OSTI ID:10175480
An AU-rich element in the 3{prime} untranslated region of the spinach chloroplast petD gene participates in sequence-specific RNA-protein complex formation
Journal Article
·
Fri Mar 31 23:00:00 EST 1995
· Molecular and Cellular Biology
·
OSTI ID:183231
Related Subjects
09 BIOMASS FUELS
090800 -- Biomass Fuels-- Production-- (1990-)
550200* -- Biochemistry
550400 -- Genetics
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BIOTECHNOLOGY
DAYS LIVING RADIOISOTOPES
DOCUMENT TYPES
FRACTIONATION
GENE MUTATIONS
GENETIC ENGINEERING
ISOTOPES
LIGHT NUCLEI
MOLECULAR BIOLOGY
MUTATIONS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
PROGRESS REPORT
PROTEIN ENGINEERING
PROTEINS
RADIOISOTOPES
RNA PROCESSING
SEPARATION PROCESSES
090800 -- Biomass Fuels-- Production-- (1990-)
550200* -- Biochemistry
550400 -- Genetics
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BIOTECHNOLOGY
DAYS LIVING RADIOISOTOPES
DOCUMENT TYPES
FRACTIONATION
GENE MUTATIONS
GENETIC ENGINEERING
ISOTOPES
LIGHT NUCLEI
MOLECULAR BIOLOGY
MUTATIONS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
PROGRESS REPORT
PROTEIN ENGINEERING
PROTEINS
RADIOISOTOPES
RNA PROCESSING
SEPARATION PROCESSES