Proliferative response of type 2 lung epithelial cells after x rays and fission neutrons

Journal Article · Sun Jun 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575322· OSTI ID:5129227

Meyer, K R; Witschi, H; Ullrich, R L

A method has been developed for study of the type 2 epithelial cell population in situ in BALB/c mouse lung by measurement of the uptake of (/sup 14/C)thymidine into DNA of pulmonary cells after stimulation with the antioxidant butylated hydroxytoluene (BHT). Within 2 days of BHT injection there was a marked increase in total lung weight, total labeling index, and (/sup 14/C)thymidine incorporation. Autoradiographic procedures revealed that the proliferative response 2 days after BHT was due primarily to type 2 alveolar cells, which comprised 80% of the (/sup 3/H)thymidine-labeled lung cells at that time. Therefore it appeared that this system might provide a means of monitoring the type 2 cell population after irradiation. Radiation modified the BHT-induced proliferative response in a dose-dependent manner, but it did not alter the relative number of labeled cell types in the lung. Thus 2 days after mice were treated with irradiation and BHT, when the proliferative response was due to type 2 cells, (/sup 14/C)-thymidine incorporation and DNA levels in the lung were determined after various doses of 300-kVp x rays and fission neutrons. Dose-response curves were generated from these data, and the radiation response was characterized by the reciprocal of the slope of the exponential region of the curves, designated D/sub 0/(PF). When irradiation was followed immediately by the proliferative stimulus BHT, the D/sub 0/(PF) values were 120 rad for 300-kVp x rays and 60 rad for fission neutrons. Based on these D/sub 0/(PF) values, relative biological effectiveness of 2.0 was obtained.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5129227
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 82:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIOXIDANTS
AZINES
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBON 14
CARBON ISOTOPES
CELL PROLIFERATION
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
EPITHELIUM
EVEN-EVEN NUCLEI
FERMIONS
FISSION NEUTRONS
HADRONS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LUNGS
MAMMALS
MICE
NEUTRONS
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
THYMIDINE
TISSUES
UPTAKE
VERTEBRATES
X RADIATION
YEARS LIVING RADIOISOTOPES