NK-2. 1: an NK-associated antigen detected with NZB anti-BALB/c serum

Journal Article · Mon Nov 01 04:00:00 EST 1982 · J. Immunol.; (United States)

OSTI ID:5127294

Pollack, S B; Emmons, S L

NZB/B1NJ mice immunized with BALB/c spleen cells produced antibodies to an NK-associated antigen(s). Antisera without detectable autoantibody lysed fewer than 5% of BALB/c spleen cells (SC) but mediated C-dependent depletion of Nk cell activity, as measured in a /sup 51/Cr-released assay with YAC-1 targets. To determine if NZB anti-BALB/c antibodies recognized an allele of the previously described locus NK-1, 136 (BALB/c x NZB)F/sub 2/ progeny were screened for sensitivity to NZB anti-BALB/c serum and anti-NK 1.1 (BALB/c x C3H F/sub 1/ anti-CE serum) and C. Segregation analysis of anti-sera sensitivity and coat color phenotype of 63 (BALB/c x NZB)F/sub 2/ indicated that sensitivity to NZB anti-BALB/c serum was linked to the agouti(a) locus on chromosome 2. Based on these data, the authors designate the antigen detected by NZB anti-BALB/c serum as NK-2 and the activity of the serum as anti-NK-2.1. Eighteen strains were tested for reactivity with anti-NK 2.1 and anti-NK-1.1. Nine were NK-1.1/sup +/, NK-2.1/sup -/, six were NK-1.1/sup -/, NK-2.1/sup +/. NK-2.1/sup +/ cells, selected with the fluorescene-activated cell sorter, were enriched in NK activity compared to NK-2.1/sup -/ SC.

• Research Organization: Univ. of Washington School of Medicine, Seattle
• DOE Contract Number: AT06-79EV10270
• OSTI ID: 5127294
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 129:5; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMMUNE REACTIONS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LYMPHOMAS
MAMMALS
MICE
NEOPLASMS
NUCLEI
PHENOTYPE
RADIOASSAY
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TUMOR CELLS
VERTEBRATES