NK-2. 1: an NK-associated antigen detected with NZB anti-BALB/c serum
NZB/B1NJ mice immunized with BALB/c spleen cells produced antibodies to an NK-associated antigen(s). Antisera without detectable autoantibody lysed fewer than 5% of BALB/c spleen cells (SC) but mediated C-dependent depletion of Nk cell activity, as measured in a /sup 51/Cr-released assay with YAC-1 targets. To determine if NZB anti-BALB/c antibodies recognized an allele of the previously described locus NK-1, 136 (BALB/c x NZB)F/sub 2/ progeny were screened for sensitivity to NZB anti-BALB/c serum and anti-NK 1.1 (BALB/c x C3H F/sub 1/ anti-CE serum) and C. Segregation analysis of anti-sera sensitivity and coat color phenotype of 63 (BALB/c x NZB)F/sub 2/ indicated that sensitivity to NZB anti-BALB/c serum was linked to the agouti(a) locus on chromosome 2. Based on these data, the authors designate the antigen detected by NZB anti-BALB/c serum as NK-2 and the activity of the serum as anti-NK-2.1. Eighteen strains were tested for reactivity with anti-NK 2.1 and anti-NK-1.1. Nine were NK-1.1/sup +/, NK-2.1/sup -/, six were NK-1.1/sup -/, NK-2.1/sup +/. NK-2.1/sup +/ cells, selected with the fluorescene-activated cell sorter, were enriched in NK activity compared to NK-2.1/sup -/ SC.
- Research Organization:
- Univ. of Washington School of Medicine, Seattle
- DOE Contract Number:
- AT06-79EV10270
- OSTI ID:
- 5127294
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 129:5; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
CHROMIUM 51
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DISEASES
ELECTRON CAPTURE RADIOISOTOPES
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