Effects of the Nd:YAG laser on DNA synthesis and collagen production in human skin fibroblast cultures
Human skin fibroblasts were subjected to treatment with a Neodymium:YAG laser at 1060 nm with varying levels of energy determined by a reproducible method of dosimetry. DNA synthesis in the cells was measured by the incorporation of (3H)thymidine, and collagen production was monitored by the synthesis of nondialyzable (3H)hydroxyproline after incubation of cells with (3H)proline. Using energy levels equal to 1.7 X 10(3) J/cm2, a significant reduction in DNA synthesis was noted, while the cells remained viable as tested by the trypan blue exclusion test. With energy levels higher or equal to 2.3 X 10(3) J/cm2, the suppression of DNA synthesis was accompanied by cell nonviability. The collagen production, when measured immediately following the treatment with 1.7 X 10(3) J/cm2, was markedly reduced, and similar effects were observed with higher energy levels. However, when the cells were tested for collagen production at 20 hours following laser treatment, there was a significant decrease in collagen production at energy levels as low as 1.1 X 10(3) J/cm2, a dose that did not affect DNA synthesis or cell viability. Thus, the results indicate that the Nd:YAG laser can selectively suppress collagen production without affecting cell proliferation. These observations suggest that laser treatment could potentially be used to reduce collagen deposition in conditions such as keloids and hypertrophic scars.
- Research Organization:
- Harbor-UCLA Medical Center, Torrance
- OSTI ID:
- 5125470
- Journal Information:
- Ann. Plast. Surg.; (United States), Vol. 11:3
- Country of Publication:
- United States
- Language:
- English
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DNA
BIOSYNTHESIS
HYDROXYPROLINE
NEODYMIUM LASERS
BIOLOGICAL EFFECTS
COLLAGEN
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
MAN
SKIN
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
LABELLED COMPOUNDS
LASERS
MAMMALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PROTEINS
PYRROLES
PYRROLIDINES
SCLEROPROTEINS
SOLID STATE LASERS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560400* - Other Environmental Pollutant Effects