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Muscarinic receptor size on smooth muscle cells and membranes

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5121027
; ;
The loss of (/sup 3/H)quinuclidinyl benzilate ((/sup 3/H)QNB) binding following high-energy radiation was used to compare the muscarinic receptor size on single smooth muscle cells isolated by collagenase digestion from the canine stomach and on plasma membranes derived from intact gastric smooth muscle without exposure to exogenous proteolysis. Radiation inactivation of galactose oxidase (68 kdaltons), yeast alcohol dehydrogenase (160 kdaltons), and pyruvate kinase (224 kdaltons) activities were used as molecular-weight standards. Radiation inactivation of (/sup 3/H)QNB binding to rat brain membranes, which gave a target size of 86 kdaltons, served as an additional control. In isolated smooth muscle cells, the calculated size of the muscarinic receptor was 80 +/- 8 kdaltons. In contrast, in a smooth muscle enriched plasma membrane preparation, muscarinic receptor size was significantly smaller at 45 +/- 3 kdaltons. Larger molecular sizes were obtained either in the presence of protease inhibitors (62 +/- 4 kdaltons) or by using a crude membrane preparation of gastric smooth muscle 86 +/- 7 kdaltons).
Research Organization:
McMaster Univ., Hamilton, Ontario
OSTI ID:
5121027
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BENZILIC ACID
CARBOXYLIC ACIDS
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DOGS
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
HYDROXY ACIDS
INACTIVATION
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PARASYMPATHOMIMETICS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RECEPTORS
SIZE
TRITIUM COMPOUNDS
VERTEBRATES