Molecular mechanism of the effects of guanine nucleotide and sulfhydryl reagent on muscarinic receptors in smooth muscles studied by radiation inactivation

Journal Article · Thu Jul 01 00:00:00 EDT 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90579-3· OSTI ID:7071875

Uchida, S; Matsumoto, K; Takeyasu, K; Higuchi, H; Yoshida, H

The molecular sizes of the units concerned in 3-quinuclidinyl benzilate (QNB) binding and in the effects of guanine nucleotide and sulfhydryl reagent on the inhibition of QNB binding by carbachol in smooth muscle of guinea pig ileum were determined to be 76,000, 179,000 and 107,000, respectively by the radiation inactivation method. One or more subunits (GTP subunit) other than the receptor subunit in a muscarinic receptor appeared to be involved in the effect of guanine nucleotide. When guanine nucleotide was present, the receptor subunit seemed to be dissociated from the GTP subunit.

• Research Organization: Osaka Univ. School of Medicine, Japan
• OSTI ID: 7071875
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:3; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BENZILIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BINDING ENERGY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
COBALT 60
COBALT ISOTOPES
DIGESTIVE SYSTEM
ENERGY
EXTERNAL IRRADIATION
GASTROINTESTINAL TRACT
GUANYLIC ACID
GUINEA PIGS
HYDROXY ACIDS
INACTIVATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INTESTINES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MOLECULAR STRUCTURE
MUSCLES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
RECEPTORS
RODENTS
SIZING
SMALL INTESTINE
THIOLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES